Fig 1.
A) Culture Protocol: B) Average tetanus force of all control donors. Significant difference seen between 0 (VC) and 5μM of statin concentration. * = P = 0.012 {Data are reported as mean ±SEM, n = 3–4 biological replicates per condition with 14 control donors} C) Average tetanus force of all case donors. Significant difference is seen between 0(VC) and 5μM of statin concentration. * = P = 0.040 {Data are reported as mean ±SEM, n = 3–4 biological replicates per condition with 12 case donors}.
Fig 2.
A) Predicted average tetanus values vs actual values for average of tetanus values from all donors in protocol II. B) P values of the individual and interaction effects.
Fig 3.
Representative immunofluorescence images of donors along with their tetanus force production.
Yellow Arrows (Striated Fiber), Blue Arrows (Fragmented Fibers). A) Striated muscle fibers (control donor dosed with simvastatin) are correlated with higher force production when compared to B) Case donor dosed with simvastatin which had reduced number of straited fibers. Increased fragmented fibers are seen in C) control donor and less striated fibers are seen in D) case donor dosed with Atorvastatin which is correlated to their tetanus force production.
Fig 4.
A) Positive correlation between striated fibers/total number of nuclei in image field and tetanus force (P = 0.015). B) Negative correlation between fragmented fibers/number of nuclei in fragmented fibers and tetanus force (P = 0.051).