Table 1.
Gender, age, and location of the German Shepherds enrolled in this study.
Fig 1.
Electrophoretic (agarose gel) fragment patterns for SOD1 (SOD: c.118G> A) mutation associated with CDM in German Shepherd dogs.
The first lane corresponds to a 100bp ladder (Ludwig Biotecnologia LTDA). The second lane corresponds to the Swiss Shepherd dog previously confirmed as AA homozygous used as a reference in this study. The third and fourth lanes correspond to GG homozygous individuals, and the fifth and sixth lanes correspond to AG heterozygous individuals. The original image was spliced to evidence the different genotype patterns; the black vertical lines represent spliced regions. The original image is presented as S1 Fig.
Fig 2.
Electropherograms of the exon 2 of the SOD1 gene region harboring the c.118G > A mutation associated with CDM.
A pink rectangle is highlighting the SNP position. The first individual (PC01) is an AA homozygous Swiss Shepherd dog used as a reference. The second (PA17) is a GG homozygous individual, and the third is a AG heterozygous individual.
Table 2.
Genotype and allele frequencies for the mutation of the SOD1 gene (SOD: c.118G > A) associated with CDM in German Shepherd dogs.
Table 3.
Reported frequency of the “A” allele in breeds predisposed to canine degenerative myelopathy.
Fig 3.
Electropherograms of the intron 1 of the SOD1 gene region harboring the newly identified ENSCAFG00000008859:g.26540247del.
A pink arrow is highlighting the deletion position. The first individual (PA71) is homozygous “TT” and used as a reference. The second to fifth individuals (PA09, PA12, PA19, and PA24) are “T-” heterozygous individuals.