Fig 1.
Example of the method 1 of sample strategy (M1).
Free-hand ROI was drawn including as much hepatic parenchyma as possible. This was repeated on 10 consecutive slices. In this example cat, at T1, the PDFF was 8.44%. Bar = 1 cm.
Fig 2.
Example of the method 2 of sample strategy (M2).
The liver parenchyma was divided in sectors. One round ROI was drawn in the central region (solid line) and 2 in the periphery (dashed lines). This was repeated on 8 slices. In this example cat, at T1, the PDFF in the central region was 9.16%, and in the periphery 7.24 and 7.56%. Bar = 1 cm.
Fig 3.
Example of the method 3 of sample strategy (M3).
ROIs were drawn on different hepatic lobes. The following hepatic lobes were considered: Caudate lobe (dotted line); right lateral liver lobe (dashed-and-dotted line); papillary process (solid line) and left lateral liver lobe (dashed line). In this example cat, at T1, the PDFF was 2.31% in the caudate liver lobe, 4.38% in the papillary process, 2.06% in the left lateral, and 3.19% in the right lateral. Bar = 1 cm.
Fig 4.
Example of the method 4 of the sample strategy (M4).
Large (at least 1cm2) ROI was drawn in the right cranial hepatic parenchyma. ROIs of similar size were drawn in the right caudal, middle and left parenchyma. In this example cat, at T1, the PDFF in the right cranial hepatic parenchyma was 4.8%. Bar = 1 cm.
Fig 5.
Example of the method 5 of the sample strategy (M5).
Small (approximately 0.5 cm2 ROIs were drawn throughout the hepatic parenchyma for a total of 16 ROIs. 2 ROIs are imaged. In this example cat, at T1, the PDFF in the imaged 2 ROIs was 7.04% and 10.4%. Bar = 1 cm.
Table 1.
Mean ± SD HFF in % with the 5 different methods on the 3 time points.
Fig 6.
Box plots of the mean HFF measured with the different 5 methods.
HFF is reported in % on x-axis. On the y-axis, the different methods of measurements are reported at the different time points: M1, M2, M3, M4, M5 at T0 with white boxes, at T1 with gray boxes, and at T2 with black boxes. For each plot, the box represents the 25th to 75th percentiles, and the dark line represents the median. Whiskers represent the highest case within 1.5-times the interquartile range and the lowest case within 1.5-times the interquartile range. Circles represent the outliers, stars extreme outliers.