Fig 1.
Transmission electron micrograph of Au-NPs.
Table 1.
Dose response percentages of Au-NP in the studied groups.
Table 2.
Primer sequences used for RT-qPCR.
Fig 2.
Cumulative mortality percentage in the control group and the Au-NPs treated group till 30 days.
Data are expressed as mean ± SE.
Fig 3.
Energy-dispersive X-ray spectra revealing the qualitative elemental composition as measured in counts per second in ovarian tissues of a: Control group and b: Au-NPs treated group.
Horizontal scale, X-ray energy; vertical scale, X-ray counts. Nd: not detected.
Fig 4.
a: Activities of antioxidant enzymes (GPOX and GSTP) (nmol NADPH/min/mg. protein) in the ovarian tissues of the control group and the Au-NPs treated group, b: Relative gene expression of miRNA-282, and c: Relative gene expression of miRNA-989 in the ovarian tissues of the control group and the Au-NPs treated group (expression was measured relative to U6 small nuclear RNA (RNU6B) using qPCR).
Data are expressed as mean ± SE.
Fig 5.
a: TUNEL assay for detection apoptosis in the ovarian tissues of beetles. Under fluorescent microscopy light green: normal DNA (non-apoptotic cell) and bright green: damaged DNA (apoptotic cell) (× 100). b: Apoptotic rate in the ovarian tissues of the control group and the Au-NPs treated group. Data are expressed as mean ± SE.
Fig 6.
Flow cytometry analysis of annexin-V-FITC and propidium iodide staining of ovarian cells in the control group (a) and Au-NPs treated group (b). The upper left (UL) quadrant (PI+/Annexin V−) represents necrotic cells, the left lower (LL) quadrant (PI−/Annexin V−) represents healthy cells, the upper right (UR) quadrant (PI+/Annexin V+) represents early apoptotic cells and the lower right (LR) quadrant (PI−/Annexin V+) represents late apoptotic cells. Apoptosis was calculated as summation of UR + LR. Values represent average percentage (± SE) of at least three samples. c: Annexin V levels in the ovarian tissues of the control group and the Au-NPs treated group. Data are expressed as the mean ± SE.
Fig 7.
Electron micrographs a: Trophocytes in the ovary of the control group showing heterochromatic (HC) Nucleus (N), nuclear envelope (Ne), mitochondria (M), rough endoplasmic reticulum (RER), and free ribosomes (r). Interstitial cells (IC). b: Trophocytes in the ovary of Au-NPs treated group showing pyknotic nuclei (arrow) and disintegrated mitochondria (M). Note: degenerated Interstitial cells (IC) (curved arrow).
Fig 8.
Electron micrographs in the oocyte of the control group and Au-Nps treated group.
a, b: Follicular epithelial cells (FEC) and oocyte in the control group. c, d, & e: Follicular epithelial cells (FEC) and oocyte in the ovary of the Au-NPs treated group. c: intended nuclear envelope (Ne), vacuolated cytoplasm (V), distorted microvilli (MV), and vacuolated ooplasm (V). d: abnormal nuclei (N) of the follicular epithelial cells (FEC) with undefined nuclear envelopes (Ne), disintegrated mitochondria (M), dilated smooth endoplasmic reticulum (SER), and vacuolated cytoplasm (V). Note: distorted microvilli (MV) and degenerated yolk granules (DYG). Arrow pointed at electron dense particles. e: follicular epithelial cell (FEC) with intended nuclear envelopes (Ne), enlarged mitochondria with distorted cristae (M), dilated rough (RER) and smooth (SER) endoplasmic reticulum, and vacuolated cytoplasm (V). Arrow pointed at electron dense particles. HC: Heterochromatin, N: nucleus, Ne: nuclear envelope, M: mitochondria, SER: smooth endoplasmic reticulum, RER: rough endoplasmic reticulum, MV: Microvilli, OC: Oocyte, OP: ooplasm, YG: yolk granules, FD: fat droplets, LD: lipid droplets, Pi: pinosomes, r: free ribosomes.