Table 1.
Demographic and clinical characteristics of healthy living donors and patients with chronic kidney disease.
Table 2.
Distribution of chronic kidney disease patient samples from each renal compartment across each disease type.
Fig 1.
Tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression in Chronic Kidney Disease (CKD) and Healthy Living Donors (HLD).
(A, C, E) Tubular ACE2 mRNA expression in (A) HLD and subjects with CKD, (C) males and females with CKD, and (E) male and female HLD. (B, D, F) Glomerular ACE2 expression in (B) HLD and subjects with CKD, (D) males and females with CKD, and (F) male and female HLD. Values are the mean ± SEM (grey lines). P values were determined by Student’s t tests.
Fig 2.
Correlation of tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression with clinical variables in subjects with Chronic Kidney Disease (CKD).
(A, C, E) Tubular ACE2 mRNA expression correlations with (A) estimated glomerular filtration rate (eGFRMDRD), (C) age, and (E) body mass index (BMI) in subjects with CKD. (B, D, F) Glomerular ACE2 mRNA expression correlations with (B) eGFRMDRD, (D) age, and (F) BMI in subjects with CKD. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).
Fig 3.
Correlation of tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression with mRNA expression of inflammation genes in subjects with Chronic Kidney Disease (CKD).
(A, B) Correlation of monocyte chemoattractant protein 1 (MCP-1 or CCL2) expression with ACE2 mRNA expression in the (A) tubulointerstitium and (B) glomeruli. (C, D) Correlation of interleukin 6 (IL6) expression with ACE2 mRNA expression in the (C) tubulointerstitium and (D) glomeruli. (E, F) Correlation of tumor necrosis factor alpha (TNF) expression with ACE2 mRNA expression in the (E) tubulointerstitium and (F) glomeruli. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).
Fig 4.
Correlation of tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression with mRNA expression of fibrosis genes in subjects with Chronic Kidney Disease (CKD).
(A, B) Correlation of collagen, type I, alpha 1 (COL1A1) expression with ACE2 mRNA expression in the (A) tubulointerstitium and (B) glomeruli. (C, D) Correlation of transforming growth factor beta 1 (TGFB1) expression with ACE2 mRNA expression in the (C) tubulointerstitium and (D) glomeruli. (E, F) Correlation of fibronectin (FN1) expression with ACE2 mRNA expression in the (E) tubulointerstitium and (F) glomeruli. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).
Fig 5.
Tubular and glomerular transmembrane protease, serine 2 (TMPRSS2) mRNA expression in Chronic Kidney Disease (CKD) and Healthy Living Donors (HLD).
(A, C, E) Tubular TMPRSS2 mRNA expression in (A) HLD and subjects with CKD, (C) males and females with CKD, and (E) male and female HLD. (B, D, F) Glomerular TMPRSS2 expression in (B) HLD and subjects with CKD, (D) males and females with CKD, and (F) male and female HLD. Values are the mean ± SEM (grey lines). P values were determined by Student’s t tests.
Fig 6.
Correlation of tubular and glomerular transmembrane protease, serine 2 (TMPRSS2) mRNA expression with clinical variables in subjects with Chronic Kidney Disease (CKD).
(A, C, E) Tubular TMPRSS2 mRNA expression correlations with (A) estimated glomerular filtration rate (eGFRMDRD), (C) age, and (E) body mass index (BMI) in subjects with CKD. (B, D, F). Glomerular TMPRSS2 mRNA expression correlations with (B) eGFRMDRD, (D) age, and (F) BMI in subjects with CKD. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).
Fig 7.
Correlation of tubular and glomerular transmembrane protease, serine 2 (TMPRSS2) mRNA expression with ACE2 mRNA levels in subjects with Chronic Kidney Disease (CKD).
(A) Tubular TMPRSS2 mRNA expression correlation with ACE2 mRNA expression. (B) Glomerular TMPRSS2 mRNA expression correlation with ACE2 mRNA expression. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).
Table 3.
Demographic and clinical characteristics of healthy living donors and subjects with diabetic nephropathy.
Fig 8.
Tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression in Diabetic Nephropathy (DN) and Healthy Living Donors (HLD).
(A, C) Tubular ACE2 mRNA expression in (A) HLD and subjects with DN, and (C) male and female subjects with DN. (B, D) Glomerular ACE2 mRNA expression in (B) HLD and subjects with DN, and (D) male and female subjects with DN. Values are the mean ± SEM (grey lines). P values were determined by Student’s t tests.
Fig 9.
Correlation of tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression with clinical variables in subjects with Diabetic Nephropathy (DN).
(A, C, E) Tubular ACE2 mRNA expression correlations with (A) estimated glomerular filtration rate (eGFRMDRD), (C) age, and (E) body mass index (BMI) in subjects with DN. (B, D, F) Glomerular ACE2 mRNA expression correlations with (B) eGFRMDRD, (D) age, and (F) BMI in subjects with DN. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).
Fig 10.
Correlation of tubular and glomerular Angiotensin-Converting Enzyme 2 (ACE2) mRNA expression with mRNA expression of inflammation and fibrosis genes in subjects with Diabetic Nephropathy (DN).
(A, B) Correlation of monocyte chemoattractant protein 1 (MCP-1 or CCL2) expression with ACE2 mRNA expression in the (A) tubulointerstitium and (B) glomeruli. (C, D) Correlation of tumor necrosis factor alpha (TNF) expression with ACE2 mRNA expression in the (C) tubulointerstitium and (D) glomeruli. (E, F) Correlation of collagen, type I, alpha 1 (COL1A1) expression with ACE2 mRNA expression in the (E) tubulointerstitium and (F) glomeruli. (G, H) Correlation of transforming growth factor beta 1 (TGFB1) expression with ACE2 mRNA expression in the (G) tubulointerstitium and (H) glomeruli. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines).