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Fig 1.

Heart rate during interventions.

Boxplots showing the heart rate of (left) privately owned horses (PRI) and (right) university horses (UNI) during baseline, social isolation, and transportation.

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Table 1.

Facial expressions during the interventions as defined by the HFI method.

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Table 1 Expand

Table 2.

Facial expressions during the interventions (combined) as defined by the Co-occurrence method.

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Table 2 Expand

Fig 2.

Frequency of EquiFACS codes.

Changes in action unit (AU) and action descriptor (AD) frequency patterns between interventions and control. Asterisk marks significant difference from control (*p<0.05, **p<0.01; ***p<0.001).

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Fig 2 Expand

Fig 3.

Maximum duration of EquiFACS codes.

The interventions affected the duration (s) of activity for an action unit (AU). Asterisk marks significant difference from control (*p<0.05, **p<0.01; ***p<0.001).

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Fig 3 Expand

Table 3.

Results of Leave-One-Out classification for action units with and without pre-selection.

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Table 3 Expand

Fig 4.

Illustration of facial expressions during the interventions.

Action units (AU)/action descriptors (AD) relevant for the interventions. A: Upper lid raiser (AU5) and Eye white increase (AD1). B: Inner brow raiser (AU101). C: Nostril dilator (AD38). D: “Ear flicker”/ Ear rotator (EAD104). E: Tongue show (AD19). Action codes (right) are compared with a “neutral” horse (left). Illustration by Anders Rådén/ARDI.

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Fig 4 Expand