Table 1.
Cytokine concentration of patient-derived (OvC3, OvC4, PeC) and artificial ascites (ArA).
Fig 1.
Cell viability and IL-6 secretion of ascites-exposed MeT-5A cells.
a) LDH absorbance of cells exposed to ascites, untreated cells, and cells exposed to the positive control Triton™ X-100 (background subtracted); * p-value < 0.0001. b) IL-6 secretion of cells exposed to ascites, normalized to untreated samples (dotted lines); * p-value < 0.05. Data are presented as median ± Min/Max, n = 3.
Table 2.
Binary logarithm of the ratio of treated to untreated cells.
Fig 2.
Permeability and F-actin texture analysis of MeT-5A cells.
a) Permeability of exposed and EDTA treated (positive control) cells normalized to untreated cells (dotted line). Data are presented as median ± Min/Max, n = 3. b) PCA of F-actin texture showing the first two PCs with the highest variance. c) Example of GLCM of untreated and OvC4 treated MeT-5A cells. Scale bar is 20 μm.
Fig 3.
Hyaluronic acid concentration of exposed MeT-5A cells compared to untreated cells.
a) HA secretion of cells exposed to ascites normalized to untreated samples (dotted line); data are presented as median ± Min/Max, n = 3. b) Fluorescence images of untreated MeT-5A cells and exposed to PeC. Cell nuclei are displayed in cyan, F-actin in magenta, and HA in green. Scale bar is 20 μm.
Fig 4.
Microvillus expression of MeT-5A cells exposed to ascites compared to untreated cells.
a) Number of microvilli of cells exposed to ascites normalized to untreated cells (dotted line); data are presented as median ± Min/Max, n = 3. b) Exemplary SEM images of microvilli, scale bars are 2 μm.
Fig 5.
SKOV-3 cell adhesion on exposed MeT-5A cells compared to untreated cells.
a) Percentage area of SKOV-3 cell adhesion on mesothelial cells when normalized to untreated cells (dotted line); data are presented as median ± Min/Max, n = 2. b) SKOV-3 cell (green) adhesion on and through the MeT-5A cell layer (magenta, exposed to OvC4), scale bar is 50 μm.