Fig 1.
Jasmonic acid (JA) and jasmonic acid isoleucine conjugate (JA-Ile) dynamical content in tomato within 12 h of grafting.
(A) Tomato hypocotyl 12 h after grafting. The top part is the scion, and the bottom part is the rootstock. The sampling distance is 5 mm above and below the joint site. Bar = 5 mm. (B) JA content changed dynamically within 12 h of grafting. (C) JA-Ile content changed dynamically within 12 h of grafting. (B-C) Coordinate axis: X axis is the time point after graft or separated; Y axis is the hormone level. Separated: hypocotyl cut off without grafting; Grafted: grafting after hypocotyl cutting; Bottom: rootstock that was grafted or bottom not grafted; Top: scion that was grafted or top not grafted. Values are expressed as the mean ± SE of three independent. Each time points compared with 0 h control, *P ≤ 0.05 and **P ≤ 0.01 in one-tail Student’s t-test (no mark was P > 0.05, the difference was not significant). Comparison between each group; Those marked with different letters were P ≤ 0.05, has a significant difference in one-tail Student’s t-test (Contains the same letter as P>0.05, the difference is not significant). Dotted line: hormone content at 0 h.
Fig 2.
Dynamic changes in the expression of JA -related genes within 12 h of grafting.
The separated part was used as control. Values are expressed as the mean ± SE of three independent replicates. Coordinate axis: X axis is the time point after graft or separated; Y axis is the gene relative expression level. Separated: hypocotyl cut off without grafting; Grafted: grafting after hypocotyl cutting; Bottom: rootstock that was grafted or bottom not grafted; Top: scion that was grafted or top not grafted. Each time points compared with 0 h control, *P ≤ 0.05 and **P ≤ 0.01 in one-tail Student’s t-test (no mark was P > 0.05, the difference was not significant). Grafted part compared with separated part control; “ab” P ≤ 0.05; “AB” P ≤ 0.01 in one-tail Student’s t-test (no mark was P>0.05, the difference is not significant). Dotted line: gene relative expression level at 0 h.
Table 1.
Symmetry of the expression patterns of JA synthesis-related genes in the top (scion) and bottom (rootstock) of tomato hypocotyls within 12 h of grafting.
Table 2.
Symmetry of the expression patterns of JA signaling genes in the top (scion) and bottom (rootstock) of tomato hypocotyls within 12 h of grafting.
Table 3.
Symmetry of the expression patterns of defense genes in the top (scion) and bottom (rootstock) of tomato hypocotyls within 12 h of grafting.
Table 4.
Symmetry of the expression patterns of JA signal regulated genes in the top (scion) and bottom (rootstock) of tomato hypocotyl after grafting within 12 h.