Table 1.
Clinical characteristics of psoriasis patients (n = 5) and volunteers (n = 5) participated in the LC-MS/MS study.
Table 2.
Clinical characteristics of psoriasis patients (n = 15) and volunteers (n = 10) participated in the ELISA assays.
Fig 1.
Venn diagram comparing DEPs in skin samples obtained from lesional and normally looking skin of the same psoriasis patients (n = 5) andskin of the healthy volunteers (n = 5) as assessed by LC/MS-MS analysis.
The numbers indicated in the diagram are the numbers of DEPs in compared groups of samples (FDR < 0.05, fold change >1.5).
Table 3.
The most abundant proteins of lesional skin.
Fig 2.
Heat map representing three clusters of DEPs involved in mRNA translation, according to the average quantitative expression levels in two groups of samples.
a) Ribosome proteins; b) Aminoacyl-tRNA synthetases; c) Translation initiation elongation and termination factors; From left to right column–the results of comparative analysis for normally looking skin of the patients vs. skin of the healthy volunteers, lesional vs. normally looking skin of the patients, and lesional skin of the patients vs. skin of the healthy volunteers. Red color indicates low expression levels whereas green color indicates high expression levels. The DEPs uniprot ID, official gene symbols of the encoding genes, fold changes and Benjamini corrected P-values are shown in S2 Table in S1 File.
Fig 3.
Heat map representation of the differentially expressed subunits of proteasome and immunoproteasome, according to the average quantitative expression levels in two groups of samples.
From left to right column–the results of comparative analysis for normally looking skin of the patients vs. skin of the healthy volunteers, lesional vs. normally looking skin of the patients, and lesional skin of the patients vs. skin of the healthy volunteers. Red color indicates low expression levels whereas green color indicates high expression levels. The DEPs uniprot ID, official gene symbols of the encoding genes, fold changes and Benjamini corrected P-values are shown in S2 Table in S1 File.
Fig 4.
Heat map showing mean expression profiles of the DEPs associated with membrane-bound vesicles, according to the average quantitative expression levels in two groups of samples.
From left to right column–the results of comparative analysis for normally looking skin of the patients vs. skin of the healthy volunteers, lesional vs. normally looking skin of the patients, and lesional skin of the patients vs. skin of the healthy volunteers. Red color indicates low expression levels whereas green color indicates high expression levels. The DEPs uniprot ID, official gene symbols of the encoding genes, fold changes and Benjamini corrected P-values are shown in S2 Table in S1 File.
Fig 5.
Heat map for the expression levels of the DEPs that function as chaperones in the cell.
From left to right column–the results of comparative analysis for normally looking skin of the patients vs. skin of the healthy volunteers, lesional vs. normally looking skin of the patients, and lesional skin of the patients vs. skin of the healthy volunteers. Red color indicates low expression levels whereas green color indicates high expression levels. The DEPs uniprot ID, official gene symbols of the encoding genes, fold changes and Benjamini corrected P-values are shown in S2 Table in S1 File.
Fig 6.
Heat map for the expression levels of DEPs capable of ATP binding.
A–proteins constituting ATP synthase and ATPases. B.–RNA helicases. From left to right column–the results of comparative analysis for normally looking skin of the patients vs. skin of the healthy volunteers, lesional vs. normally looking skin of the patients, and lesional skin of the patients vs. skin of the healthy volunteers. Red color indicates low expression levels whereas green color indicates high expression levels. The DEPs uniprot ID, official gene symbols of the encoding genes, fold changes and Benjamini corrected P-values are shown in S2 Table in S1 File.