Table 1.
Reference of Metarhizium species used in phylogenetic analyses, showing their culture number, isolation source and country of origin, and the GenBank accession numbers of the translation elongation factor 1 alpha (TEF-1α), the beta-tubulin (BtuB) genes and the internal transcribed spacer (ITS) region.
Fig 1.
Morphology of Metarhizium majus TH152, (A) a three-week-old culture on SDAY medium; (B) spores on older plates; (C) bundles of spore strings of the same length; (D) spore shape and size; (E, F) mature phialides with conidiogenous cells and conidia; (G, H) scanning electron microscope picture showing the surface of the conidia. (Scale bars: A = 2 mm; B = 500 μm, C = 5 μm; D-F = 10 μm; G: 10 μm).
Fig 2.
The neighbour-joining likelihood phylogenetic tree generated using PAUP with uncorrected “p” option, of Metarhizium majus related to the PARB and MGT clades from the analysis of the datasets of 5’intron-rich region of the translation elongation factor 1 alpha (TEF-1α).
Bootstrap values/Bayesian probabilities are denoted above the branch. The tree was rooted, using the sequence from Metarhizium brasilense ARSEF2948T as outgroup; the isolates with T = indicate the type strain.
Fig 3.
Neighbour-joining phylogenetic tree generated using PAUP with uncorrected “p” option, of Metarhizium majus with regards to related species, based on analysis of the 5’intron-rich region of the translation elongation factor 1 alpha (TEF-1α) gene sequences concatenated with the internal transcribed spacer (ITS) region and the beta-tubulin (BtuB) gene.
The tree was rooted using the sequence from Metarhizium frigidum ARSEF 4124T as the outgroup. Bootstrap values/Bayesian probabilities are denoted above the branch; isolates with T = indicate the type strain.
Table 2.
Estimates of evolutionary divergence between type strains of the translation elongation factor 1 alpha (TEF-1α) gene of different Metarhizium species.
The number of base pairs difference between the sequences is shown in the form of a matrix, with the standard error above the diagonal. Evolutionary analyses were done in Mega 7.