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Fig 1.

Sampling sites: a, b, c = lagoons 1, 2, and 3 (State Morrope); and d = lagoon 4 (State Bayovar).

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Table 1.

Data related to saline lagoon samples.

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Table 1 Expand

Table 2.

16S rRNA gene-based identification of bacteria isolated from lagoons Morrope and Bayovar.

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Table 2 Expand

Fig 2.

Phylogenetic tree based on 16S rRNA gene sequences (1206–1500 bp positions in the final dataset) inferred using the neighbor-joining method in MEGA7, the evolutionary distances were computed using the Kimura 2-parameter method, showing the phylogenetic positions of different saline isolates and type strains within the Streptomyces and Pseudonocardia genera.

Numbers at branching points refer to percentages of bootstrap values from 1000 replicates. Bar, 0.02 substitutions per nucleotide position was used. Bacillus cereus ATCC 14579T (AE016877) was the outgroup.

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Table 3.

Minimum inhibitory concentration of three Streptomyces sp. crude extracts, as determined by broth dilution method.

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Table 4.

Antiproliferative activity of three Streptomyces extracts against human tumor cell lines.

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Table 5.

Antiproliferative evaluation of isolate Streptomyces sp. MW562807 fermented in three different media and fractions of the R2A medium against nine tumors and one non-tumor cell line.

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Fig 3.

Molecular networking obtained for Streptomyces sp. MW562807 extract Cholic Acid and its derivatives were grouped in cluster A, and Lobophorins were grouped in cluster B.

Nodes circled in blue indicate molecules identified by comparison with the GNPS platform database.

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Table 6.

MS data obtained for secondary metabolites detected in Streptomyces sp. MW562807 extract.

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Table 6 Expand

Fig 4.

Structures of secondary metabolites identified in Streptomyces sp. MW562807 extract.

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