Fig 1.
The different rates of cells containing refractile bodies that were distributed to the ICM.
The relationship between the order of cell division and ICM allocation is shown. The first 50% of cleaved blastomeres during the fourth division had significantly higher rates of being incorporated in the ICM (p<0.001).
Table 1.
Univariate and multivariate logistic regression analyses with the attainment of ICM defined as the endpoint.
Fig 2.
Dendrogram and the average order in which the cells cleaved during the 3rd and 4th divisions in each cluster.
The dendrogram revealed that samples can be divided into two clusters, according to the order of cell division. Cluster analysis showed that blastomeres which cleaved earlier tended to reach the ICM.
Fig 3.
Example of blastomere distribution to the ICM or TE obtained by tracking the position of refractile bodies.
In these sequential photographs, the refractile bodies were distributed to the ICM (Fig 3) or the TE (Fig 4). The white arrows indicate the large refractile bodies in 2PN zygotes and 2-cell, 4-cell, 8-cell, morula, and blastocyst stage embryos. The numbers indicate the time in hours from ICSI.
Fig 4.
Example of blastomere distribution to the ICM or TE obtained by tracking the position of refractile bodies.
In these sequential photographs, the refractile bodies were distributed to the ICM (Fig 3) or the TE (Fig 4). The white arrows indicate the large refractile bodies in 2PN zygotes and 2-cell, 4-cell, 8-cell, morula, and blastocyst stage embryos. The numbers indicate the time in hours from ICSI.