Table 1.
Descriptive statistics of the six traits for parents and across BC2F4 derived lines.
Fig 1.
Correlation of delayed leaf senescence with agronomical traits in the BC2F4 derived population.
** Correlation is significant at the 0.01 level (2-tailed); *Correlation is significant at the 0.05 level (2-tailed).
Fig 2.
SNP- based genetic map and distribution of QTLs associated with delayed leaf senescence traits.
Chromosomes are numbered at the top and QTLs are listed on the right of each chromosome. Various color figures indicate the location of peak LODs of the six traits. QTL detected at heading are marked as * and 30 days after heading are marked as **.
Table 2.
QTL identification for the six delayed leaf senescence traits in BC2F4 derived lines.
Table 3.
List of five candidate genes identified in four genomic regions.
Fig 3.
Expression of five genes (1) Os01t0743200 (Pectinesterase) (2) Os02t0511100 (Oxidoreductase, short chain dehydrogenase/reductase) (3) Os06t0590301 (short-chain dehydrogenase/reductase) (4) Os06t0653100 (Senescence-associated protein (5) Os10t0419600 (Chlorophyllase family protein) in flag and second leaf of Swarna (less senescent) and Moroberekan (more senescent) along with two early senescence and two delayed senescence lines. Bold letter represents higher expression and a normal letter represents less expression. Heatmap of six genes in flag and second leaf of Swarna, Moroberekan and phenotypic extreme BC2F4 derived lines viz., ELS-early leaf senescence and DLS- delayed leaf senescence. Expression is shown as log2 (FC) relative to second leaf of early senescence parent. Red marks genes that are upregulated and green for downregulated. The y-axis shows genes that co-regulate based on their directionality and magnitude. The x-axis shows different according to the legend.