Fig 1.
3E11 mouse monoclonal antibodies against the unique C-terminus of LaNt α31 validation.
(A) Diagram of the laminin and laminin-related protein architecture. (B) Multiple sequence alignment of LaNt α31 C-terminus with equivalent regions of laminin N-terminal sequences. Peptide unique to LaNt α31 used to generate antibodies highlighted by red box. (C) Paraffin-embedded human tongue tissue sections processed for immunohistochemistry with rabbit polyclonal antibodies against LaNt α31 or mouse monoclonal antibodies against LaNt α31 (3E11). Scale bars represent 200 μm or 100 μm in magnified images. (D) Immunoblot of total protein from hTCEpi expressing either: shRNAs specific to LaNt α31, scrambled shRNA controls, or full-length LaNt α31-PAmCherry cDNA. Ponceau S stained membrane below. (E) Immunoblot of dilution series of recombinant MBP-LaNt α31, and MBP-netrin-4 probed with 3E11 antibodies. Diagram below shows the structure of the recombinant MBP-LaNt α31 and Netrin-4. (F) ELISA using recombinant MBP-LaNt α31, LM511, or LM332. Wells were coated overnight, blocked with BSA, and then probed with 3E11 antibodies. Bound 3E11 antibodies were detected with HRP-conjugated secondary antibodies, HRP-substrate added and absorbance read at 450 nm.
Fig 2.
LaNt α31 is enriched in a sub-population of basal epidermal keratinocytes.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11, A), laminin α3 (B), or mouse IgG (C). Scale bars represent 100 μm or 50 μm in magnified images. Arrowheads in (A) indicate localised enrichment of LaNt α31. Arrowheads in (B) indicate basement membrane reactivity for laminin α3.
Fig 3.
LaNt α31 is expressed throughout the blood vasculature.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11). Representative images taken from: (A) breast, (B) colon, (C) small intestine, (D) oesophagus. Scale bars represent 60 μm.
Fig 4.
LaNt α31 is enriched in the ECM surround terminal ducts in breast tissue.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11, A), laminin α3 (B), or mouse IgG (C). Arrowheads denote matrix immunoreactivity surrounding ducts, chevrons indicate lobular acini. Scale bars represent 200 μm or 100 μm in magnified images.
Fig 5.
LaNt α31 localises to the alveolar basement membranes in lung tissue.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11, A), laminin α3 (B), or mouse IgG (C). Arrowheads depicts basement membrane, chevrons indicate reticular fibre-like immunoreactivity. Scale bars represent 200 μm or 100 μm in magnified images.
Fig 6.
LaNt α31 is expressed throughout the epithelium of the digestive tract.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11), laminin α3, or mouse IgG. (A) oesophagus, (B) stomach, (C) small intestine, colon (D), rectum (E). Arrowheads in (A) indicate basement membrane immunoreactivity. Scale bars represent 100 μm or 50 μm in magnified images.
Fig 7.
LaNt α31 expression in kidney, pancreas, liver, and spleen, is largely similar to that of LMα3.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11), laminin α3, or mouse IgG. (A) kidney, (B) liver, (C) pancreas, (D) spleen. Arrowheads in (A) depict ducts displaying high immunoreactivity, chevrons indicate parietal epithelium of the Bowman’s capsule. Arrowheads in (B) indicate branches of the bile ducts. Arrowheads in (D) indicate splenic cords of Billroth. Scale bars represent 100 μm or 50 μm in magnified images.
Fig 8.
LaNt α31 is expressed throughout the female reproductive system.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11), laminin α3, or mouse IgG. (A) ovary, (B) fallopian tube, (C) endometrium, (D) cervix. Arrowheads in (A) indicate follicular cells of the primordial follicles, chevrons indicate primary follicles, and double chevrons indicate vasculature. Arrowheads in (B) indicate peg cells, chevrons indicate ciliated cells. Arrowheads in (C) indicates uterine glands of the endometrium. Arrowheads in (D) indicate reticular fibre-like immunoreactivity. Scale bars represent 100 μm or 50 μm in magnified images.
Fig 9.
LaNt α31 is expressed in the testis and prostrate.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11), laminin α3, or mouse IgG. (A) testis, (B) prostrate. Arrowheads in (A) indicate a sub-population of the spermatogonia, chevrons indicate Sertoli cells. Arrowheads in (B) indicate basal layer of the acini, chevron indicate acinar cells. Scale bars represent 100 μm or 50 μm in magnified images.
Fig 10.
LaNt α31 is expressed by neurons in the central nervous system.
Paraffin-embedded human tissue microarray sections processed for immunohistochemistry with mouse monoclonal antibodies against LaNt α31 (3E11), laminin α3, or mouse IgG. (A) cerebellum, (B) cerebral cortex, (C) spinal cord. Arrowheads in (A) indicate Purkinje neurons in the ganglionic layer, chevrons indicate axons throughout granular layer. Arrowheads in (B) indicate cell bodies towards the axon hillock of pyramidal cells, chevrons indicate glial cells. Arrowheads in (C) indicate motor neurons. Scale bars represent 100 μm or 50 μm in magnified images.