Fig 1.
Alignment of replicase proteins present in different grapevine vitiviruses.
Multiple amino acid sequences available in GenBank were aligned to identify a conserved region among the viruses (GVA to GVM). Consensus and identity are displayed at the top of the alignment. Left column corresponds to GenBank accession numbers. Identified motifs (A and B) are indicated in blue color.
Fig 2.
Detection of different grapevine vitiviruses by reverse transcription PCR using degenerate primers.
Lane 1, grapevine virus A; lane 2, grapevine virus B; lane 3, grapevine virus D; lane 4, grapevine virus E; lane 5, grapevine virus F; lane 6, grapevine virus G; lane 7, grapevine virus H; lane 8, grapevine virus I; lane 9, grapevine virus J; lane 10, grapevine virus L; lane 11, grapevine virus M; lane 12, grapevine Pinot gris virus; lane 13, grapevine rupestris stem pitting-associated virus; lane 14, healthy grapevine; lane M, 1 Kb Plus DNA Ladder marker. Expected amplicon size: 219 bp.
Fig 3.
Detection of a dilution series of grapevine viruses A and B (GVA and GVB) by universal and GVA or GVB specific assays.
OC, original concentration; total RNA diluted in water from 10−1 to 10−5.
Fig 4.
Analysis of different hosts infected by vitiviruses using the universal assay.
Lane 1, blueberry infected by blueberry green mosaic-associated virus; lane 2, mint infected by mint virus 2; lane 3, grapevine infected by grapevine virus A; lane 4, healthy grapevine; lane M, 1 Kb Plus DNA Ladder marker.
Table 1.
BLASTp analysis of amino acid (aa) sequences, motif A and motif B, present in different vitiviruses.