Fig 1.
Workflow in the RNAthor system.
Fig 2.
Scheme of the RNAthor algorithm for unreliable data exclusion.
Fig 3.
Automatic and manual normalization of RNA probing data.
SHAPE (A) and DMS (C) reactivity profiles calculated by RNAthor (red) and manually (black). Correlation between RNAthor and manual analysis per nucleotide reactivity estimated for SHAPE (B) and DMS (D).
Table 1.
The results of validation of RNAthor algorithm for unreliable data identification.
Fig 4.
Example verification of the repeatability of RNA chemical probing experiments.
Comparative step plot for repeatable (A) and non-repeatable (B) replicates of Ty1 RNA probing with DMS. (C) The box-and-whisker plot and violin plot presenting the differences in reactivity data distribution obtained for repeatable and non-repeatable experiments.