Fig 1.
Alcohol dependent and non-dependent mice present with significant differences in immune cell compositions and cytokine expression.
(A-C) Immune cell expression as percentages of all CD45+ cells in (A) blood, (B) spleen and (C) liver isolated from alcohol dependent (red, solid fill) and non-dependent (black outline, white fill) mice. (D-F) Expression of cytokines in (D) blood, (E) spleen and (F) liver CD45+ cells isolated from dependent and non-dependent mice. (G) CD4+ and CD8+ T cell expression as a percentage of CD3+ cells isolated from spleen of dependent and non-dependent mice. (H) Th1 (CD4+ IFN-γ+), Th2 (CD4+ IL-4+), Th17 (CD4+ IL-17+), and Treg (CD4+ FoxP3+) expression as a percentage of CD4+ T helper cells isolated from liver of dependent and non-dependent mice.*, p<0.05; **, p<0.01 analyzed by Mann-Whitney U test; n = 5–8.
Fig 2.
IFN-γ expression is increased in alcohol dependent compared to control mice.
(A-G) Expression of IFN-γ by (A) B cells, (B) NK cells, (C) T cells, (D) NKT cells, (E) macrophages, (F) neutrophils and (G) dendritic cells in alcohol dependent (red, solid fill) and non-dependent (black outline, white fill) mice. (H-J) Immune cells as percentages of all IFN-γ producing CD45+ cells in (H) blood, (I) spleen and (J) liver isolated from dependent and non-dependent mice. *, p<0.05; **, p<0.01 analyzed by Mann-Whitney U test; n = 5–8.
Fig 3.
IL-17 expression is increased in alcohol dependent compared to control mice.
(A-G) Expression of IL-17 by (A) B cells, (B) NK cells, (C) T cells, (D) NKT cells, (E) macrophages, (F) neutrophils and (G) dendritic cells in alcohol dependent (red, solid fill) and non-dependent (black outline, white fill) mice. (H-J) Immune cells as percentages of all IL-17 producing CD45+ cells in (H) blood, (I) spleen and (J) liver isolated from dependent and non-dependent mice. *, p<0.05; **, p<0.01 analyzed by Mann-Whitney U test; n = 5–8.
Fig 4.
Immune cells co-expressing IFN-γ and IL-17 are significantly increased in alcohol dependent mice.
(A) Co-expression of IFN-γ and IL-17 in blood, spleen, and liver CD45+ cells isolated from alcohol dependent (red, solid fill) and non-dependent (black outline, white fill) mice. (B-H) Expression of IL-17 by (B) B cells, (C) NK cells, (D) T cells, (E) NKT cells, (F) macrophages, (G) neutrophils and (H) dendritic cells in dependent and non-dependent mice. (I-K) Immune cells as percentages of all IFN-γ+ IL-17γ+ CD45+ cells in (I) blood, (J) spleen and (K) liver isolated from dependent and non-dependent mice. *, p<0.05; **, p<0.01 analyzed by Mann-Whitney U test; n = 5–8.