Fig 1.
Representative immunofluorescent staining of ICSI zygotes of the indicated groups.
(A) Homospecific porcine-porcine zygote showing asymmetric SMARCA4 levels between PN. (B) Homospecific porcine-porcine zygote showing similar SMARCA4 levels between PN. (C) Heterospecific zebra-porcine zygote. (D) Heterospecific horse-porcine zygote. Scale bars indicate 50 μm.
Fig 2.
Analysis of embryo quality and SMARCA4, YAP1 and SOX2 expression.
(A) Total cell number in the blastocysts from the indicated groups. (B) Percentage of YAP1-positive (YAP1+) nuclei in blastocysts from the indicated groups. (C) Percentage of SOX2-positive (SOX2+) nuclei in blastocysts from the indicated groups. (D) Percentage of YAP1+ and SOX2+ nuclei in blastocysts from the indicated groups. (E) Immunofluorescence quantification (arbitrary units) of SMARCA4 in ICSI zygotes of the indicated groups. (F) Total cell number in ICSI blastocysts from the indicated groups. Errors bars in all panels display standard error of the mean (SEM).
Table 1.
Pronuclear evaluation of zebra, horse and porcine sperm cells injected into matured porcine oocytes.
Table 2.
In vitro preimplantation development of zebroid and horse ICSI embryos.
Fig 3.
Representative immunofluorescent staining of ICSI blastocyst stage embryos of the indicated groups.
Scale bars indicate 50 μm.
Table 3.
In vitro preimplantation development of zebra and horse SCNT embryos.
Fig 4.
Representative immunofluorescent staining of SCNT blastocyst stage embryos of the indicated groups.
Scale bars indicate 50 μm.