Fig 1.
Representative putative microplastics from environmental samples photographed with a stereo-zoom microscope/camera after their collection from surface waters of the Elizabeth River at Old Dominion University’s Sailing Center.
Samples were collected from June through November 2015. Most pieces were distinctly biofouled. Bars represent one millimeter.
Table 1.
Identification of 23 putative microplastic pieces using ATR-FTIR.
Fig 2.
Concentrations of putative Vibrio spp. on PE (environmental samples; green triangles) and in paired water samples (filled blue circles).
Fig 3.
A. Colonization Experiment #1 (12 Oct– 10 Nov 2015): Mean (n = 3, ± 1 sd) concentrations of putative Vibrio spp. from biofilms on plastics and glass, and from paired water samples. Water temperature ranged from 15–22°C. For clarity, points are jittered about the day of sampling. Values for day 1 all were zero and are not displayed. Substrate types: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP), polycarbonate (PC), and glass (Glass). B. Colonization Experiment #2 (12 Jan– 10 Feb 2016): Mean (+ 1 sd) concentrations of putative Vibrio spp. from biofilms on plastics and glass, and from paired water samples. Water temperature ranged from 2.1–9.5°C. Note the decreased range of the Y-axis relative to Fig 3A. For clarity, points are jittered about the day of sampling. Values for day 1 all were zero and are not displayed. Polystyrene (PS) was added to the list of substrates referenced in Fig 3A.
Table 2.
Vibrio spp. isolates (n = 76) resistant, intermediate, or susceptible to ampicillin (AM), streptomycin (S), rifampin (RA), tetracycline (TE), gentamicin (GM), and chloramphenicol (C).
Values are shown for number of isolates and corresponding percentage (in parentheses). Bolding indicates the most common forms of resistance. A) V. parahaemolyticus, B) V. cholerae, C) V. vulnificus.
Fig 4.
Hierarchical clustering dendrogram showing isolates’ relationship based on their antibiotic susceptibility profiles.
ZOI data for each isolate was compared with that of all other isolates using Euclidean distance similarity, then clustered using a group average algorithm. Red symbols, Colonization Experiment #1; blue symbols, Colonization Experiment #2; green symbols, environmental samples. Plastic substrates (all types), triangles; glass substrate; +; water, filled circles.
Fig 5.
Principal components analysis (PCA) of antibiotic profiles of Vibrio spp. (n = 97) by sample type.
PC1 represents increasing susceptibility to streptomycin (S), rifampin (RA), and tetracycline (TE), and PC2 represents increasing susceptibility to chloramphenicol (C) and gentamicin (GM). Eigenvectors for each antibiotic are shown as lines adjacent to the corresponding labels. Symbols as in Fig 4. The PCA is overlain with Euclidean distance (value of 3) from the cluster analysis (Fig 4).
Fig 6.
Principal components analysis (PCA) of antibiotic profiles identifying the 97 Vibrio isolates.
PC1 and PC2 as in Fig 5. Eigenvectors for each antibiotic are shown as lines. Isolates are color coded: magenta, V. vulnificus; blue, V. parahaemolyticus; green, V. cholerae.
Fig 7.
Relative abundances of sequenced Bacteria in Colonization Experiment #1.
Substrate types: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP), polycarbonate (PC), and glass (G). Paired water samples were not collected. The most abundant OTUs are listed as follows: (1) Bacteria, Bacteria Unclassified; (2) Bacteria, Proteobacteria, Gammaproteobacteria, Pseudomonadales, Pseudomonadaceae, Pseudomonas; (3) Bacteria, Proteobacteria, Gammaproteobacteria, Oceanospirillales, Oceanospirillaceae; (4) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Tenacibaculum; (5) Bacteria, Proteobacteria, Gammaproteobacteria, Pseudomonadales, Pseudomonadaceae, Pseudomonas; (6) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Maribacter; (7) Bacteria, Verrucomicrobia, Verrucomicrobiae, Verrucomicrobiales, Rubritaleaceae, Rubritalea; (8) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Flavobacterium; (9) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Pseudoalteromonadaceae, Pseudoalteromonas; (10) Bacteria, Proteobacteria, Gammaproteobacteria, Oceanospirillales, Oceanospirillaceae, Oleibacter; (11) Bacteria, Actinobacteria, Actinobacteria, Actinomycetales, Corynebacteriaceae, Corynebacterium; (12) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae; (13) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae; (14) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Alteromonadaceae, Glaciecola; (15) Bacteria, Bacteria Unclassified; (16) Bacteria, Proteobacteria, Gammaproteobacteria, Vibrionales, Vibrionaceae, Vibrio; (17) Bacteria, Proteobacteria, Alphaproteobacteria, Sphingomonadales, Sphingomonadaceae, Sphingomonas; (18) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Polaribacter; (19) Bacteria, Proteobacteria, Alphaproteobacteria, Sphingomonadales, Erythrobacteraceae, Erythrobacter; (20) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae; (21) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae.
Fig 8.
Relative abundances of sequenced Bacteria in Colonization Experiment #2.
As in Fig 7, with the addition of polystyrene (PS) as a substrate and paired water samples. The most abundant OTUs are listed as follows: (1) Bacteria, Proteobacteria, Gammaproteobacteria, Oceanospirillales, Oceanospirillaceae; (2) Bacteria, Bacteria Unclassified; (3) Bacteria, Proteobacteria, Alphaproteobacteria, Sphingomonadales, Sphingomonadaceae, Sphingomonas; (4) Bacteria, Bacteria Unclassified; (5) Bacteria,Verrucomicrobia, Verrucomicrobiae, Verrucomicrobiales, Verrucomicrobiaceae, Luteolibacter; (6) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Flavobacterium, (7) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, (8) Bacteria, Proteobacteria, Betaproteobacteria, Burkholderiales, Comamonadaceae, Delflia, (9) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae, Loktanella; (10) Bacteria, Proteobacteria, Gammaproteobacteria, Oceanospirillales, Oceanospirillaceae; (11) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Tenacibaculum, (12) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Polaribacter; (13) Bacteria, Proteobacteria, Betaproteobacteria, Burkholderiales, Comamonadaceae; (14) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Flavobacterium; (15) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Pseudoalteromonadaceae, Pseudoalteromonas, (16) Bacteria, Proteobacteria, Gammaproteobacteria, Pseudomonadales, Pseudomonadaceae, Pseudomonas; (17) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Maribacter; (18) Bacteria, Bacteria Unclassified; 19) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae; (20) Bacteria, Proteobacteria, Gammaproteobacteria, Vibrionales, Vibrionaceae, Vibrio; (21) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Alteromonadaceae, Glaciecola; (22) Bacteria, Actinobacteria, Actinobacteria, Actinomycetales, Corynebacteriaceae, Corynebacterium, (23) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Maribacter; (24) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae.
Fig 9.
Relative abundances of sequenced Bacteria on polyethylene microplastics (ATR-FTIR confirmed) and paired water samples.
Sample number is indicated as AL##. The most abundant OTUs are listed as follows: (1) Bacteria, Proteobacteria, Gammaproteobacteria, Oceanospirillales, Oceanospirillaceae; (2) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Alteromonadaceae, Glaciecola; (3) Bacteria, Proteobacteria, Gammaproteobacteria, Pseudomonadales, Pseudomonadaceae, Pseudomonas; (4) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Pseudoalteromonadaceae, Pseudoalteromonas; (5) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Cryomorphaceae, Lishizhenia; (6) Bacteria, Proteobacteria, Gammaproteobacteria, Alteromonadales, Alteromonadaceae, Glaciecola; (7) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae, Pseudoruegeria; (8) Bacteria, Proteobacteria, Gammaproteobacteria, Vibrionales, Vibrionaceae, Vibrio; (9) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Maribacter; (10) Bacteria, Verrucomicrobia, Verrucomicrobiae, Verrucomicrobiales, Rubritaleaceae, Rubritalea; (11) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Flavobacterium; (12) Bacteria, Proteobacteria, Gammaproteobacteria, Pseudomonadales, Pseudomonadaceae, Pseudomonas; (13) Bacteria, Proteobacteria, Betaproteobacteria, Betaproteobacteria Unclassified; (14) Bacteria, Proteobacteria, Betaproteobacteria, Burkholderiales, Comamonadaceae; (15) Bacteria, Verrucomicrobia, Verrucomicrobiae, Verrucomicrobiales, Verrucomicrobiaceae, Luteolibacter; (16) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae; (17) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Tenacibaculum; (18) Bacteria, Bacteroidetes, Flavobacteria, Flavobacteriales, Flavobacteriaceae, Krokinobacter; (19) Bacteria, Bacteria Unclassified; (20) Bacteria, Actinobacteria, Actinobacteria, Acidimicrobiales, Acidimicrobiaceae, Ilumatobacter; (21) Bacteria, Proteobacteria, Alphaproteobacteria, Rhodobacterales, Rhodobacteraceae.
Fig 10.
Hierarchical clustering dendrogram of bacterial communities showing sample relationship based on sequence data.
Abundance data (Log (x+1)) was compared between samples using Bray-Curtis similarity, then clustered using a group-average algorithm. A) Colonization Experiment #1; B) Colonization Experiment #2; C) environmental samples (polyethylene microplastics and paired water samples). Substrate types: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP), polycarbonate (PC), glass (Glass), and polystyrene (PS).