Fig 1.
Custom-made 3D printed bone plate.
(A) The shape of the designed bone plate. (B) The custom-made 3D printed bone plate. The plate was designed to contact the bone only via the parts of the screw holes. The screw holes were designed as a hemispherical shape fit for the screw head.
Fig 2.
Representative craniocaudal and mediolateral radiographic views before and after surgery.
Radiographs of forelimb taken pre-surgery, post-surgery (day 0), and 1, 3, 5 and 7 months after the operation.
Fig 3.
Radiographs of forelimb with a bone plate at 7 months after the operation.
An arrow indicates the osteotomized position.
Fig 4.
μCT examination of the removed radii.
(A) Positions at which μCT images were taken. (B) μCT cross-sections at each position, 7 months after the operation.
Fig 5.
Method for analyzing apatite c-axis orientation.
(A) Beam path of X-ray. (B) μXRD patterns (Debye rings); the vertical direction (upper and lower parts) corresponds to the radial long axis.
Fig 6.
BMD and preferential apatite c-axis orientation of the radii analyzed at the position shown in Fig 4A.
(A) Variation in BMD and (B) variation in the degree of preferential apatite c-axis orientation along the radial long axis as a function of bone position. Gray and white symbols represent the value in the intact (free from stress shielding) and regenerated positions, respectively. a: P < 0.05 vs position 5. b: P < 0.05 vs position 10.
Fig 7.
Schematic drawing showing the variations in the apatite orientation and BMD in the intact, in the stress shielded, and in the regenerated portions under stress shielding conditions.