Fig 1.
α: α-secretase. sAPPα: soluble APPα fragment. β: β-secretase. sAPPβ: soluble APPβ fragment. γ: γ-secretase. AICD: APP Intracellular domain.
Fig 2.
Concentrations of Aβ40 and Aβ42 in the neuroblastoma supernatant using the MSD Elisa test.
The neuroblastoma transfection with a wild APP plasmid increased the concentrations of Aβ40 and Aβ42 peptides in the cell culture medium. Statistic test: Two-way ANOVA Sidak’s multiple comparisons test. P value style **** p<0.0001. n.d: not detectable.
Fig 3.
Aβ peptide concentrations in culture medium.
Neuroblastomas were transfected with plasmids coding for 30 FAD mutations with or without an additional Icelandic (A673T) mutation (except for A673V). Aβ40 concentration (A) and Aβ42 concentration (B) when various FAD mutations are present in exon 16. Aβ40 concentration (C) and Aβ42 concentration (D) when various FAD mutations are present in exon 17. Statistical test: two-way ANOVA Sidak’s multiple comparisons test (n = 6). P value style * p<0.0332, ** p<0.0021, *** p<0.0002, **** p<0.0001.
Fig 4.
Variations of Aβ40 and Aβ42 peptide concentrations with A673T mutation.
Percentage changes of Aβ40 and Aβ42 peptide concentrations induced by the addition of the A673T mutation to plasmids containing or not an FAD mutation. * indicates this variation was statistically significant with the two-way ANOVA Sidak’s multiple comparisons test.
Fig 5.
Culture medium of cells transfected with plasmids coding for 30 FAD mutations with or without an additional Icelandic (A673T) mutation (except for A673V).
Table 1.
Percentage variations in Aβ40 and Aβ42 peptide concentrations due to the A673T insertion.
Fig 6.
Aβ peptide concentrations with wild type or London APP gene with or without A673T mutation.
Aβ40 (in A) and Aβ42 (in B) concentrations for neuroblastomas transfected with plasmids coding either for a wild type APP gene or an APP gene with the London mutation (V717I) with and without the Icelandic (A673T) mutation. Statistic test: Two-way ANOVA Sidak’s multiple comparison test (n = 6). P value style * p<0.0332, ** p<0.0021, *** p<0.0002, **** p<0.0001.