Fig 1.
Flowchart for bioinformatics search for candidate salivary miRNA markers in periodontal diseases and diabetes mellitus.
Fig 2.
Venn diagram of bioinformatics search for candidate miRNA markers in periodontitis and diabetes mellitus.
Table 1.
Demographic data of the studied sample.
Fig 3.
The expression of miRNA a) miR-155, b) miR-146b, c) miR-146a and d) miR-203 in the study groups. Data shown is the standardized Ct for each miR in each participant of the study (n = 53). The horizontal line represents the mean Ct value for each group. Ct is inversely proportional to the gene expression; thus, the lower the CT value, the higher the expression level.
Table 2.
The difference between the four study groups in standardized Ct value (ΔCt) for miRNA-155, miRNA 146b, miRNA 146a and miRNA 203.
Fig 4.
ROC curve for selected markers when used as test to predict a diagnosis of DM among subjects with healthy gum (smaller values of the marker are predictive of a positive diagnosis).
Table 3.
Area under ROC curve (AUC) for selected markers when used as a test to predict a diagnosis of diabetes among subjects with healthy periodontium, predict periodontitis among subjects with diabetes, predict periodontitis among subjects with no diabetes, or predict diabetes among subjects with periodontitis.
Table 4.
Validity parameters for selected markers when used as a test to predict a diagnosis of diabetes among subjects with healthy periodontium (smaller values of the marker are predictive of a positive diagnosis).
Table 5.
Validity parameters for selected markers when used as test to predict periodontitis among subjects with diabetes.
Fig 5.
ROC curve for selected markers when used as test to predict periodontitis among subjects with no DM (smaller values of the marker are predictive of a positive diagnosis).
Table 6.
Validity parameters for selected markers when used as a test to predict periodontitis among subjects with no diabetes (smaller values of the marker are predictive of a positive diagnosis).
Table 7.
Validity parameters for selected markers when used as a test to predict a diagnosis of diabetes among subjects with periodontitis.
Fig 6.
Mir 146a/b and Mir 155 regulatory network in diabetes mellitus and periodontitis.