Fig 1.
The relative electrical conductivity (REC), respiration rate and weight loss rate (a, b and c) of passion fruit with 1-MCP and PF treatment during storage stages at room temperature (22–25°C).
Antioxidant enzymes activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) (d, e and f), and activities of β-galactosidase (β-Gal), polygalacturonase (PG) and cellulase (g, h and k) in control, 1-MCP and PF-treated passion fruit during storage stages at room temperature (22–25°C). Values are the means ± SD.
Fig 2.
Phenotype images of ‘Tainong1’ purple passion fruits during ripening stage and effects of PF and 1-MCP treatments on the passion fruit at postharvest storage, including a (4 weeks post anthesis), b (6 weeks post anthesis), c (8 weeks post anthesis—physiological maturity), d (store at room temperature for 4 days), e (store at room temperature for 8 days), f (PF treatment for 4 days), g (PF treatment for 8 days), h (1-MCP treatment for 4 days) and j (1-MCP treatment for 8 days).
Fig 3.
The number of unigenes annotation in GO, KO, KOG, NR, NT, PFAM and Swiss-port database by RNA_seq data analysis (a). KEGG classification (b) and Gene ontology (GO) functional classifications (c) of all expressed genes detected by RNA-Seq in passion fruit at all stages (ripening and storage stage).
Fig 4.
The expression pattern of Differentially Expressed Genes (DEGs) and KEGG enriched pathway in purple passion fruits during ripening stage.
(a) Venn diagram of DEGs in C vs A and B vs A comparison groups. (b) Top 20 KEGG enrichment pathways in passion fruit during ripening stage. Rich factor was represent the degree of DEGs enrichment in this pathway.
Fig 5.
Expression profile of DEGs expression related to flavonoids and anthocyanin synthesis (a), synthesis of carotenoids (b) and phenylpropanoid biosynthesis pathway (c).
Red indicates up-regulated and blue was down-regulated DEGs expression in heatmap. Red border in Fig 5c indicates up-regulated DEGs, green border was down-regulated DEGs and yellow border was both up- and down-regulated DEGs. The corresponding index are listed in SF1 and SF2 in S1 File. Data are presented as the mean± SD of three biological replicates.
Fig 6.
Top 20 KEGG enrichment pathways in passion fruit during postharvest stage.
(a) The DEGs enriched KEGG pathway in E vs C. (b) The DEGs enriched KEGG pathway in G vs E. (c) The DEGs enriched KEGG pathway in J vs E. C (8 weeks post anthesis—physiological maturity), E (store at room temperature for 8 days), G (PF treatment for 8 days), and J (1-MCP treatment for 8 days). Rich factor was represent the degree of DEGs enrichment in this pathway.
Fig 7.
The heat-map of DEGs expression profile related to starch and sucrose metabolism (a) and plant hormone signal transduction (b) in passion fruit during postharvest storage with different treatment. Red indicates up-regulated and blue was down-regulated DEGs expression. A (4 weeks post anthesis), B (6 weeks post anthesis), C (8 weeks post anthesis—physiological maturity), E (store at room temperature for 8 days), G (PF treatment for 8 days), and J (1-MCP treatment for 8 days).
The corresponding index are listed in SF3 and SF4 in S1 File. Data are presented as the mean ± SD of three biological replicates.