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Fig 1.

Experimental design and sample collection (A) and small RNA profiles in sweet cherry scions (B).

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Table 1.

Summary of small RNA sequencing reads.

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Table 2.

Metrics of the representative transcriptome of sweet cherry (Prunus avium L. 'Tieton').

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Table 3.

The number and fraction (%) of sRNAs in sweet cherry scions and potential scion-to-rootstock transferred sRNAs.

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Fig 2.

Summary of sRNAs (A) and gene networks of overrepresented sRNAs in bud tissues of sweet cherry scion 15 (Scion15) ‘Emperor Francis’ grafted on a ‘Gisela 6’ rootstock (RS15). The ontology file of GO_FULL in BiNGO and A. thaliana annotation were used as the references to identify overexpressed GO terms (P < 0.05). Bubble color indicates the P-value. Overrepresented sRNAs in “Biological process” (B) and “Molecular function” (C). No overrepresented GO terms are present in “Cellular component”.

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Fig 3.

A diagram showing the pipeline of determining scion-to-rootstock transferred sRNAs.

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Fig 4.

Profiles of putative sRNAs transferred from sweet cherry scions to rootstocks.

RS15_specific and RS19_specific are sRNAs found in the grafted rootstocks, RS15 and RS19, but absent in the ungrafted.

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