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Fig 1.

Transmission electron micrographs of the four S. agalactiae phages (LF1 –LF4).

Scale bar of 50 nm included in each micrograph.

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Table 1.

S. agalactiae phages LF1, LF2, LF3 and LF4 virion measurements*.

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Table 1 Expand

Fig 2.

Representative examples of the spot test outcomes including plaques, clear, turbid and negative.

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Fig 3.

The relationship between S. agalactiae host serotype and LF1 (panel A, n = 125/256), LF2 (panel B, n = 91/256), LF3 (panel C, n = 75/256) and LF4 (panel D, n = 151/256) phage activity presented as the number of isolates that were sensitive (plaques, black; clearing, light grey; and turbidity, dark grey) and their serotype.

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Table 2.

The host range activity of four S. agalactiae phages against clinical carriage (antenatal) and disease (neonatal) S. agalactiae isolates (n = 256).

The activity is divided into plaque formation, clear lysis zones, turbid zones and no activity.

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Fig 4.

The organisation of genes in the genomes of four S. agalactiae phages with modules highlighted.

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Fig 5.

Comparative alignment of all S. agalactiae phages demonstrating the percentage nucleotide similarity of the different genomes (colour scale percentage similarity).

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Fig 6.

The neighbour-joining phylogenetic tree analysis of LF1 –LF4 phages (purple) and 263 complete Streptococcus phage genomes and their respective viral families.

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Fig 6 Expand