Fig 1.
Flow chart showing the pipeline used for the identification and characterisation of miRNA-derived SSRs in common wheat.
Fig 2.
Physical locations of the 13 selected miRNAs on 11 different wheat chromosomes.
Table 1.
Details of 13 selected miRNA-SSRs, including gene names, sequences, miRNA lengths, pre-miRNA lengths, and chromosomal locations or genomic coordinates.
Fig 3.
PAGE profile of SSR marker ‘HT-160b’ on 37 wheat genotypes showing length polymorphism.
M = 100 base pair ladder, 1–26; heat tolerant wheat genotypes, and 27–37; heat susceptible wheat genotypes. The arrow shows four alleles—a, b, c, and d (marked on genotypes 21–24) amplified by HT-160b.
Table 2.
Details of 13 miRNA–SSRs, including miRNA gene family, SSR motif, primer sequences, and product size.
Fig 4.
Estimation of the number of groups based on the output from the software STRUCTURE.
4a: Plot of ΔK over K (range 2–10) calculated using the data of 13 miRNA-SSRs genotyped on a set of 37 wheat genotypes. 4b: Bar plot showing the grouping of 37 genotypes into four different clusters. 4c: PCA plot showing the grouping of 37 wheat genotypes into four groups.
Table 3.
Analysis of molecular variance (AMOVA) calculated using the data of 13 miRNA-SSRs genotyped on a set of 37 wheat genotypes.
Table 4.
Genetic diversity parameters including the number of different alleles (Na), number of effective alleles (Ne), information index (I), expected heterozygosity (He), and unbiased heterozygosity (UHe) in four sub-population of the 37 genotypes.
Fig 5.
Un-weighted neighbor-joining tree showing the grouping of 37 genotypes based on miRNA-SSRs data.
The genotypes highlighted in red color are heat-susceptible, while genotypes written in black color are heat-tolerant. Values given in blue color are bootstrap values.