Table 1.
Strains and plasmids used in this study.
Table 2.
Primers used for plasmid copy number determination by quantitative PCR.
Fig 1.
Structure and organization of pRC12.
A) Physical and genetic map of plasmid pRC12 from L. curvatus CRL 705. Orientation of deduced CDSs are shown by purple arrows. B) Genetic map of plasmid pUCL287 (X75607.1) from Tetragenococcus halophilus ATCC33315. C) Structural organization of the replicative region of plasmids pRC12 and pUCL287. D) Comparison between plasmids pRC12 from L. curvatus CRL 705 and pUCL287 from Tetragenococcus halophilus ATCC33315; both plasmids share high identity of the replication region (ori-repA) and the integrase and toxin-antitoxin system.
Table 3.
Functions of pRC12 CDSs and similarities with proteins in the GenBank database.
Fig 2.
Structure and organization of pRC18.
A) Physical and genetic map of the plasmid pRC18 from L. curvatus CRL 705. Deduced CDSs are shown by purple arrows indicating their orientation. B) Focus on an 11 kb insertion disrupting repA showing the presence of transposases, and the lactocin 705 operon.
Table 4.
Functions of pRC18 CDSs and similarities of their encoded proteins present in the GenBank database.
Fig 3.
Comparison between pRC12 and pRC18 plasmids.
The grey scale indicates the identity % between nucleotide sequences. Integrase, antitoxin/ toxin, and repA (pUCL287-like; disrupted in pRC18 by an 11 kb insertion DNA element) genes are common in plasmids pRC12 and pRC18.
Fig 4.
Comparison of pRC18 structural organization with other plasmids.
GC skew (purple) and GC content (black) of pRC18 are shown in the inner circles. The pRC18 CDSs (red) are indicated and their paralogs in different plasmids are shown with a color code.
Fig 5.
Genetic context of the replication region of the plasmid pRC18.
The coding sequence of repB’ gene (which encodes a 168 aa protein) is underlined. The sequences of directed repeats (DR) and inverted repeats (IR1 and IR2) are in bold type and indicated with arrows. The predicted position of the -35, -10 boxes and the ribosome binding sites are in bold type.
Fig 6.
It was constructed by ligating a 3.4 kb HpaI-EcoRI fragment of pRC18 (coordinates nt 10334–13806) into the HindIII (blunted)-EcoRI sites of a derivative-plasmid pBlueScript II SK (+) containing the chloramphenicol (cat) gene from plasmid pC194.
Table 5.
Electrotransformation of p3B1 in different LAB hosts.