Table 1.
The reads, bases number and mapping rate of the L. edodes transcriptome.
Fig 1.
Gene ontology analysis of 2-fold upregulated genes under blue light conditions.
GO annotation was conducted using the Blast2GO software and the genes were grouped into three main GO categories including, biological process, molecular function, and cellular component.
Fig 2.
KOG functional analysis of 2-fold upregulated genes under blue light conditions.
KOG analysis was conducted using the KOG online tool and the genes were sub-grouped into cellular process and signaling, information storage and processing, metabolism, and poorly characterized genes.
Fig 3.
KEGG analysis of 2-fold upregulated genes under blue light conditions.
Fig 4.
Heat map and hierarchical clustering analysis of 4-fold up- and down-regulated genes under blue light and dark conditions.
Each column represents an experimental sample (no light and blue light), and each row represents a gene. Red and green colors indicate high and low expression of each gene, respectively. Each value on the heat map represents normalized reads count (RC) data using log2 transformation.
Fig 5.
Validation of 8 upregulated DEGs according to a 4-fold change using quantitative RT-PCR.
Each experiment was normalized by a housekeeping gene (Rpl4).
Fig 6.
Validation of 4 downregulated DEGs according to a 4-fold change using quantitative RT-PCR.
DEG39-1 and DEG39-2 represent the same cytochrome P450 gene but a different sequence. DEG39-3 represents qRT-PCR results performed with cytochrome P450 specific primers which were designed at conserved sequence regions. Each experiment was normalized by the 18S housekeeping gene.
Table 2.
Summary of blue light induced 4-fold change DEGs.
Fig 7.
Morphological transformations according to different cultivation conditions.
A, B, E, F, I, and J were cultivated under blue light. C, D, G, H, K, L were cultivated under darkness (no light). Growth stage 1, 2, and 3 represents harvest times (2, 4, and 6 days after beginning either treatment).
Fig 8.
Comparison of pileus diameter and length as well as stipe diameter and length under blue light or dark conditions during fruit development.
The black lines indicate diameter or length under blue light cultivation. Gray dashed lines indicate diameter or length under dark (no light) cultivation. Growth stage 1, 2, and 3 represents harvest times (2, 4, and 6 days after beginning either treatment).