Fig 1.
Geographic distribution of the 15 Araucaria angustifolia populations studied.
Population code used and respective locations are: 1.BAR: Barbacena–MG; 2.IPI: Ipiúna de Calda, MG; 3.CON: Congonhal, MG; 4. LAM: Lambarí, MG; 5.VAR: Vargem Grande do Sul, SP; 6.CAM: Camanducaia, MG; 7.CJO: Campos do Jordão, SP; 8.ITA: Itapeva, SP; 9.ITR: Itararé, SP; 10.IRA: Iratí, PR; 11. IRT: Iratí (Tardio), PR; 12.QBA: Quatro Barras, PR; 13.CAC: Caçador, SC; 14.CHA: Chapecó, SC; 15:TRB: Três Barras, SC. Indicated also the experimental station where the provenance/progeny field trial was established and actual samples collected for the population survey.
Table 1.
Summary of the number of single-nucleotide polymorphisms (SNPs) filtered out from each sequence source (RAD and RNA sequences) following the simultaneous filters applied for SNP selection toward the construction of the Araucaria angustifolia 3K SNP Axiom® Array.
Table 2.
Summary of performance of SNPs derived from different sources (RAD-seq and RNA-seq) according to the two different performance criteria adopted (see Methods for details).
Table 3.
Comparative summary of genetic diversity parameters (Ho observed heterozigosity; He expected heterozygosity) and inbreeding coefficient (Fis) with its respective 95% confidence interval (C.I) obtained with GDA for the two different data sets for the 15 A. angustifolia populations.
Table 4.
Comparative summary of genetic variation parameters and F-statistics of population differentiation via AMOVA (Analysis of Molecular Variance) for A. angustifolia populations using different molecular marker sets together with previously published estimates for similarly regionally located populations.
Fig 2.
Population structure analyses of the 15 A. angustifolia populations using a multidimensional principal coordinate analysis (PCoA) and different markers types (microsatellites or SNPs) and different numbers of SNPs.
The proportions of variation explained by the first three PCoA axes are indicated from left to right respectively in each plot.
Fig 3.
Comparative population structure analyses for the 15 A. angustifolia populations indicated at the bottom of each panel obtained with the software STRUCTURE using a reduced (80) and full (2,022) set of SNPs and an eight-microsatellite set for different numbers of ‘k’ clusters (K = 2 to 4).