Table 1.
Demographic, clinical and tissue-related features of cases of Cohorts I and II.
Table 2.
List of altered proteins previously reported in other brain regions in proteomic studies in schizophrenia.
Table 3.
List of proteins filtered from enriched and most representative functions in the post-mortem cerebellum in schizophrenia.
Fig 1.
Validation analysis of hit candidate proteins by immunoblot in Cohort I.
Protein extracts from samples of the post-mortem cerebellum of non-psychiatric control (C, n = 7), schizophrenia (SZ, n = 7) and non-schizophrenia suicide (n = 6) subjects (Table 1, Cohort I) were analysed by immunoblot for VPP1, PRVA, calmodulin (CaM) and GAPDH and quantified by densitometry. (A) Protein levels for each were normalized to GAPDH values and to the mean of the control samples. Each box plot represents the median, interquartile range and range of each group from at least two independent determinations. Statistical analysis was performed using the Kruskal-Wallis test for differences between groups (VPP1: p = 0.0095; PRVA: p = 0.0011; CaM: p = 0.0270) and the t test for comparison between the indicated groups. (n.s.-not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). (B) Representative Western blot images for VPP1, PRVA and GAPDH in 3 non-psychiatric control individuals, 3 schizophrenia subjects and 3 non-schizophrenia suicide controls. (C) Representative Western blot images for CaM and GAPDH in 3 non-psychiatric control individuals, 3 schizophrenia subjects and 3 non-schizophrenia suicide control. See S1 Raw images for complete western blot images.
Table 4.
Association analysis of other variables in Cohorts I and II.
Fig 2.
Validation analysis of hit candidate proteins by immunoblot in Cohort II.
Protein extracts from samples of the post-mortem cerebellum of non-suicide chronic schizophrenia (SZ, n = 13) and control (C, n = 14) subjects (Table 1, Cohort II) were analysed by immunoblot for the same proteins as in Fig 1A and quantified by densitometry. (A) Protein levels for each protein were normalized to GAPDH values and to the mean of the control samples. Each box plot represents the median, interquartile range, and range of each group from at least two independent determinations. An outlier was detected for VPP1 protein values in the control group. Statistical analysis for comparison between case and control groups was performed using the t test for VPP1 and PRVA and the Mann-Whitney U test for CaM and GAPDH (n.s.-not significant, *p<0.05, ***p<0.001). (B) Representative Western blot images for VPP1, PRVA, CaM and GAPDH in 6 non-psychiatric control individuals and 6 non-suicide schizophrenia subjects from set 2. See S2 Raw images for complete western blot images.