Fig 1.
C1 indicates plots had compost added, while C0 indicates no compost was added. Nitrogen fertilizer was added to plots in purple (200 N), or no nitrogen added was added (orange plots).
Table 1.
Average maximum and minimum daily air and soil (5cm depth) temperatures and average, maximum and minimum daily precipitation recorded at the experimental site over the sampling period.
Fig 2.
Timeline showing the soil sampling time points and when compost was added to compost plots.
Below the crops grown in each year are shown.
Fig 3.
Change in abundance of nematodes in 300 ml of soil of treatment plots over time based on morphological data.
Each bar represents the average of three replicate samples. Nematodes are classified by feeding types: bacterivores, fungivores, ectoparasitic herbivores (herbivores_e), migratory herbivores (herbivores_m), epidermal/root hair feeders (herbivores_rh), sedentary parasites (herbivores_s), omnivores and predators. Substacks indicate different taxa.
Fig 4.
Change in relative abundance of nematodes in treatment plots based on read counts of metabarcoding data from 300 ml of soil, showing the shift of relative nematode abundance and feeding type over time.
Each bar represents the average of three replicate samples. Nematodes are classified by feeding types: bacterivores, fungivores, ectoparasite herbivores (herbivores_e), migratory herbivores (herbivores_m), epidermal/root hair feeders (herbivores_rh), sedentary parasites (herbivores_s), omnivores and predators. Substacks indicate different taxa.
Table 2.
One-way ANOVA results for soil nematode abundance and trophic groups between compost, nitrogen addition, treatment, and timepoint using morphological and metabarcoding data.
Fig 5.
Maturity index of the nematode community using morphological and metabarcoding data for each treatment and timepoint.
Each bar represents the average of three replicate samples, error bars indicate the standard deviation.
Fig 6.
Heat map of average read counts of taxa present in 300 ml of compost (presence only counted if total read count was above 30 over all 5 compost samples), and soil nematodes in common with compost-present taxa.
Fig 7.
Relative abundance of the nematode community at the Family level, over all time points and treatments of nematode communities using morphological and metagenomic identification methods.
Only families found in both identification methods are shown and with greater than 0.2% relative abundance.
Fig 8.
Bray-Curtis based non-metric multi-dimensional scaling (NMDS) plots of all samples in either morphological identified samples (A) or molecular identified samples (B).