Table 1.
Overview of subprojects and animals used.
Fig 1.
Timeline of behavioral experiments performed.
Fig 2.
Correlation matrix of selected parameters.
Fig 3.
Principal component analysis of selected parameters.
A principal component analysis was performed 100 times using a resampled random subset of 80% of the total data. Overall runs PC2 was consistently able to separate the treatment groups on the basis of four parameters: saccharin preference, burrowing behavior, time spent in social interaction and distance moved in the open field. (A) One representative visualization of the principal component analysis. (B) Scatter plot of individual animals and their associated treatment group along PC1 and PC2, the size of the point indicates the number of seizures observed during a two-week monitoring phase.
Fig 4.
Individual results of selected behavioral parameters.
On the basis of the outcome of the principal component analysis, four parameters were used to create a composite score: (A) The amount of gravel burrowed in the burrowing test. (B) The distance moved in the open field. (C) Time spent in active social interaction and (D) the percentage of preference for saccharin solution of normal tap water.
Fig 5.
Total distribution of severity clusters per treatment group.