Fig 1.
Incorporation rate of each deoxyribonucleoside.
(a) Sagami Bay in July 2015. (b) Sagami Bay in January 2016. (c) Lake Kasumigaura in July 2015. (d) Lake Kasumigaura in January 2016.
Fig 2.
Ratios of incorporation rates of each deoxyribonucleoside.
(a) Sagami Bay in July 2015. (b) Sagami Bay in January 2016. (c) Lake Kasumigaura in July 2015. (d) Lake Kasumigaura in January 2016.
Fig 3.
Relationships between incorporation rates of deoxyribonucleosides.
(a) dA and dT. (b) dA and dT excluding an apparent outliner shown in (a). (c) dG and dT. (d) dC and dT. The regression lines obtained by model II linear regression were (b) [dA] = 0.87×[dT]– 2.3, (n = 13, r2 = 0.96, p < 0.001), (c) [dG] = 2.5×[dT]– 15 (n = 14, r2 = 0.92, p < 0.001) and (d) [dC] = 0.22×[dT]– 1.8 (n = 14, r2 = 0.85, p < 0.001). White and black circles represent Sagami Bay and Lake Kasumigaura, respectively. The dotted line represents 1:1 incorporation.
Table 1.
Relative incorporation rates of 15N-dA and 15N-dT, 15N-dG against that of 15N-dT.
± represents 97.5% confidence interval of the relative incorporation rates.
Fig 4.
Relationships of dT incorporation rates between samples incubated with 15N-dT and 15N-dC.
(a) Sagami Bay. (b) Lake Kasumigaura. The regression line in Lake Kasumigaura was significant, and the slope of the regression line was more than 1 ([dC→dT] = 1.5 × [dT→dT]– 2.4; n = 8, r2 = 0.92, p < 0.001). The dotted line represents 1:1 incorporation.
Fig 5.
Metabolic pathway of pyrimidine-base nucleosides and nucleotides.
dC; deoxycytidine, dCMP; deoxycytidine monophosphate, dCDP; deoxycytidine diphosphate, dCTP; deoxycytidine triphosphate, dU; deoxyuridine, dUMP; deoxyuridine monophosphate, dT; thymidine, dTMP; thymidine monophosphate, dTDP; thymidine diphosphate, dTTP; thymidine triphosphate. dC is phosphorylated by deoxycytidine kinase, and dU and dT are phosphorylated by thymidine kinase.