Table 1.
Ergot alkaloid concentration of tall fescue pastures.
Table 2.
Bacterial species richness and diversity estimators in fecal microbial communities across location1 and tolerance groups2.
Table 3.
Fungal species richness and diversity estimators for fecal microbial communities across location1 and tolerance groups2.
Fig 1.
Statistically significantly (q≤0.05) different abundant fungal OTUs considering interactions of location (BBCFL, UPRS) and tolerance group (HT, LT) as an effect where effects due location had the same direction.
The differences in abundance of fungal OTUs are shown as log2 fold changes for each tolerance interaction. Positive values represent higher abundance in HT cattle, negative values represent higher abundance in LT cattle. Bars in blue and purple represent farms BBCFL and UPRS, respectively. Error bars represent the 95% confidence interval.
Fig 2.
Statistically significantly (q≤0.05) different abundant bacterial and fungal OTUs considering location (BBCFL, UPRS) as an effect.
The differences in abundance of OTUs are shown as log2 fold changes for each location. Positive values represent higher abundance in farm BBCFL, negative values represent higher abundance in farm UPRS. Error bars represent the 95% confidence interval.
Fig 3.
Statistically significantly (q≤0.05) different abundant bacterial and fungal OTUs considering tolerance group (HT, LT) as an effect.
The differences in abundance of OTUs are shown as log2 fold changes for each tolerance group. Positive values represent higher abundance in HT cattle, negative values represent higher abundance in LT cattle. Error bars represent the 95% confidence interval.
Fig 4.
Canonical discriminant analysis (CDA) for response to fescue toxicosis.
CDA was performed to discriminate animals based on the combination between tolerance group (T; High [HT] and Low [LT] tolerance groups) and location (BBCFL and UPRS) using 14 fungal and bacterial OTUs. Each point represents the canonical score (CS) of each animal based on the respective canonical variable (CAN). The x-axis represents CAN 1 and the y-axis CAN 2. Red and Blue points represent HT and LT animals, respectively, whereas triangles and stars represent animals from UPRS and BBCFL, respectively.
Table 4.
Canonical discriminant analysis for tolerance group1 (T), Location2 (L), and interaction between T and L (T*L).