Fig 1.
Cotton leaf spot caused by A. tubingensis.
(A) NIA- Sadori (resistant variety) (B) CIM-573 (susceptible variety) (C) NIA-Sadori (mock inoculated) (D) CIM-573 (mock inoculated).
Fig 2.
Measurement of disease severity in V1 = CIM-573 variety and V2 = NIA-Sadori variety.
Fig 3.
Principal Component analysis (A) and 2D Scores plot (B) in cotton leaves under control (mock) and treated (pathogen inoculated) conditions.
Mock and treated samples formed separate groups, indicating an altered state of metabolite levels in the leaves. Slight overlapping with each other was also observed.
Fig 4.
OPLS-DA plots of four data sets: Resistant Mock vs Susceptible Mock, Resistant Treated vs Susceptible Treated, Susceptible Mock vs Susceptible Treated, Resistant Treated vs Resistant Mock.
Fig 5.
PLS-DA of four data sets: Resistant Mock vs Susceptible Mock, Resistant Treated vs Susceptible Treated, Susceptible Mock vs Susceptible Treated, Resistant Treated vs Resistant Mock.
Fig 6.
Volcano maps of four data sets: Resistant Mock vs Susceptible Mock, Resistant Treated vs Susceptible Treated, Susceptible Mock vs Susceptible Treated, Resistant Treated vs Resistant Mock.
Fig 7.
Venn diagram of differential metabolites in cotton leaf, after pathogen infection.
Fig 8.
Hierarchial clustering showing heat map of RRC metabolites with fold change > 1 in the Mock treatments (R_Mock vs. S_Mock) and their response in other treatments generated using Metaboanalyst software.
Red and green colors represent up and down regulation, respectively. Columns are exhibiting samples and rows are exhibiting metabolites here.
Fig 9.
Hierarchial clustering showing heat map of top 60 RRI metabolites of resistant and susceptible varieties, induced by the infection of A. tubingensis, generated using Metaboanalyst software.
Red and green colors represent up and down regulation, respectively. Columns are presenting samples and rows are exhibiting metabolites here. Clustering is evident from the shown dendrograms.
Table 1.
List of few significantly important metabolites (RRI and RRC) with their names, molecular formula (MF), compound type, fold change (FC) and genomes identifier number (KEGG ID/HMDB ID) in cotton leaves under control and pathogen inoculated conditions.
Table 2.
Pathway ID and names, total metabolites involved in those pathways, metabolites significantly accumulated in present study and false discovery rate (FDR) in cotton leaves of resistant and susceptible variety, identified by pathway analysis using Arabidopsis thaliana as the pathway library.