Fig 1.
FT-IR spectra (a) of aqueous P407, U, P407+U, HA, and P407+U+HA. Chemical structure of (b) P407 and (c) HA.
Fig 2.
Micellization intensity P407-based gels.
Tonset, CMT, and Tendset decreased when HA was added into the aqueous (a) 23% and (b) 25% P407. The influence of HA on micellization intensity was relatively minor. The micellization process was delayed and micellization intensity was decreased when (c) urokinase was added into aqueous P407 solution.
Fig 3.
Gel dissolution profiles of (a) 23% and (b) 25% P407 with and without HA. The axis represents residual gel weight percentage at different time points. The addition of HA into 23% and 25% P407 gels markedly increases swelling rate in the first 3 hours. The addition of HA significantly slowed down the gel erosion in 23% and 25% P407 gels when compared at (c) 20th and (d) 24th hour, respectively. Significant differences from control group (P407+U) were denoted with *P < 0.05 and **P < 0.01.
Fig 4.
Gel dissolution profiles of (a) 23% and (b) 25% urokinase-loaded P407 with and without HA.
The gel dissolution time decreased markedly in urokinase-loaded 23% and 25% P407 in the comparison with the non-urokinase-loaded gels.
Fig 5.
Urokinase release curves of 23% (a) and 25% (b) P407-based gels.
The addition of HA into 25% P407 gel significantly increased urokinase activity.
Fig 6.
SEM images of the microstructure of (a) 23% and (e) 25% P407-based gels. Urokinase was added to the (b) 23% and (f) 25% P407. A more compact and porous structure was observed with an addition of 6mg HA to the (c, g) 23% and (d, h) 25% P407.
Fig 7.
Alamar blue assay was used to determine the Hs68 cells cell viability.
Urokinase and 6mg HA were added to (a)23% P407 (b)25% 407.