Fig 1.
Manhattan plot indicating the associations between DBP and DNA methylation of men of European and South Asian ancestry.
The plot demonstrates the associations between DBP and DNA methylation of European and South Asian men collectively. The model was adjusted for confounders, estimated cell counts and principal components. The uncorrected log10(p-values) are plotted on the y-axis. The blue line is drawn to separate the CpG sites that surpassed p-value<1×10−05, a threshold for a suggestive association and the red line to separate the CpG sites that surpassed the Bonferroni-corrected threshold (p-value<1.24×10−07). The CpG sites that surpassed the Bonferroni-corrected threshold were considered to be associated with the trait.
Fig 2.
A Manhattan plot indicating the associations between DBP and DNA methylation in European men.
The plot demonstrates the associations between DBP and DNA methylation in European men. The model was adjusted for confounders, estimated cell counts and principal components. The uncorrected–log10(p-values) are plotted on the y-axis. The blue line is drawn to separate the CpG sites that surpassed p-value<1×10−05, the threshold suggestive of an association and the red line to separate the CpG sites that surpassed the Bonferroni threshold (p-value<1.24×10−07). The CpG sites that surpassed the Bonferroni threshold were considered to be associated with the trait.
Table 1.
Levels of genomic inflation (Lambda) for each EWAS comparison after correcting for confounders including cell counts.
Fig 3.
Consistency between fully adjusted EWAS of SBP, DBP and hypertension between Europeans and South Asian.
DNA methylation associations are shown for 402331 common CpG sites across three EWAS analyses. Each green dot represents a CpG site and the positions of the dots are determined by the effect size in each analysis. The grey lines on each dot denote the confidence intervals (CI) for the estimates. A linear fit of the overall correspondence summarises correlation between compared associations (green dashed line). Grey dashed line shows the line of equality in effect sizes between pairs of analyses. R2 = goodness of linear fit and as such is a measure of the consistency between two EWAS.
Table 2.
Overlap of DMRs between fully adjusted DMR analysis of SBP and DBP for trans-ancestry, Europeans and South Asians respectively.
Fig 4.
Venn diagrams to identify overlap between DMRs (mapped to genes) of DBP (A) and SBP (B) analyses conducted for South Asian and European groups.
SA denotes South Asian and Eu denotes European ancestry.
Table 3.
Distributions of study characteristics included in the EWAS analysis of SBP, DBP and hypertension.