Fig 1.
The northwestern embankment of the Tiber River along Porto di Ripa Grande.
The arrows indicate two examples of sampled areas (black arrow = B; white arrow = U).
Fig 2.
Optical microscope images of the microorganisms observed in B samples.
(A) Chroococcus litophilus (Cyanobacteria); (B) coccoid green alga (Chlorophyta); (C) meristematic fungus.
Fig 3.
Statistical analyses of 16S rRNA gene sequencing data.
(A) Beta diversity represented by Principle Coordinate Analysis Emperor plot on a Bray-Curtis distance matrix; (B) Boxplots of Alpha-diversity Index (calculated as Shannon index) in both black patina and uncolonized region samples; (C) Stacked bar charts of the taxonomic profile at family level, low abundance taxa are lumped together in the “Others” category.
Fig 4.
(A) Beta diversity represented by a Principle Coordinate Analysis Emperor plot on a Bray-Curtis distance matrix; (B) Stacked bar charts of the taxonomic profile at species level, low abundance taxa are lumped together in the “Others” category.
Fig 5.
Species found to be differentially abundant using LEfSe analysis on shotgun metagenomics data analyzed using kraken.
Table 1.
MAGs genome assembly statistics including completeness and redundancy scores.
Fig 6.
Bar charts summarizing the number of proteins annotated (y-axis) in a specific COG category (on the x) in the seven MAGs.
Fig 7.
Boxplots showing the differential abundance of specific bacterial taxa.
The graphics are divided according to the phyla they belong.
Fig 8.
Boxplots showing the differential abundance of specific fungal taxa.
The graphics are divided according to the order they belong.