Fig 1.
Images of hemangiosarcoma (HSA) tumors from dogs.
A) Primary HSA mass in a spleen. B) Metastatic HSA in a liver with multifocal masses in all lobes. C) Metastatic hemangiosarcoma in the lungs with multifocal masses in all lobes. D) Photomicrograph of a splenic HSA from a dog in the current study that was PCR positive for B. henselae. 20X magnification. Hematoxylin and eosin. Credits: Talley A (image A), Sommer S (image B), Rasche B (image C) and Barnes J (image D).
Fig 2.
Geographic distribution of dogs with HSA.
Shows the percentage of dogs positive for Bartonella DNA on PCR of HSA tumor tissue and/or non-tumor tissue biopsy. Color indicates region. Black text indicates regions from which 10 or more samples were received, and gray text indicates regions from < 10 samples were received.
Table 1.
Demographic and clinicopathologic characteristics of study population.
Median and range shown for continuous data. The * indicates a proportion significantly different from baseline (breed baseline = mixed breed).
Table 2.
Number of samples tested from each anatomic location, and number of each sample positive for each pathogen tested. Serum was tested for Bartonella spp. antibodies using IFA; serology for hemotropic Mycoplasma and Babesia spp. was not performed. All other samples were tested by PCR for DNA of each pathogen.
Fig 3.
Proportion of samples positive for Bartonella spp.
Color indicates sample type (HSA tumors, green; non-tumor tissue, pink; whole blood, purple; serum, brown; any sample, grey). A) Blood, serum, HSA tumors, and non-tumor tissues (all anatomic locations combined). Blood and tissue were tested by PCR for Bartonella spp. DNA, serum was tested by IFA for Bartonella spp. antibodies. Different superscripts indicate significantly different proportions (p < 0.05, Chi-squared test) B) Left panel shows HSA tumors by anatomic location, right panel shows non-tumor tissues by anatomic location. The * indicates a statistically significant difference in proportion from spleen samples (p < 0.05, Chi-squared or Fisher’s exact test).
Fig 4.
Proportion of dogs with HSA positive for hemotropic pathogen DNA on any sample.
* indicates statistically significant difference in proportion from Bartonella spp. (p < 0.05, Chi-squared test).