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Fig 1.

Study design.

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Fig 2.

Mutation distribution in plasma samples of 121 NSCLC patients at diagnosis.

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Fig 3.

Median allele frequency of mutations detected in tissue and plasma grouped based on their concordance among the two materials.

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Fig 4.

Comparison of the mutation distribution in plasma and tissue of 36 patients with newly diagnosed NSCLC.

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Table 1.

Sensitivity, specificity, PPV (Positive Predictive Value) and concordance for mutations detected in paired plasma and tissue biopsy analysis.

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Fig 5.

EGFR mutation distribution in ctDNA of 50 patients with an EGFR mutation at diagnosis that have relapsed following TKI treatment.

A. Analysis by cobas B. Analysis by NGS.

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Table 2.

Gene mutation results obtained by cobas and NGS methods in 50 consecutive NSCLC patients referred for T790M resistance mutation analysis due to relapse after TKI treatment.

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Fig 6.

NGS-cobas comparison in 50 EGFR mutant NSCLC patients in relapse following EGFR TKIs treatment.

EGFR sens.: EGFR sensitizing mutations in exons 18, 19, 21.

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Fig 7.

Apportionment of the 59 NSCLC patients with at least one variant identified in liquid biopsy analysis, using 4 different biomarker categories: traditional targeted treatment biomarkers (EGFR, ALK); all approved treatment associated biomarkers (EGFR, ALK, BRAF); approved and emerging treatment biomarkers (EGFR, ALK, BRAF, HER2); approved, emerging treatment & clinical trials associated biomarkers (EGFR, ALK, BRAF, HER2, KRAS, NRAS, MET, PIK3CA, STK11,TP53).

In case a patient harbors more than one mutation the categorization is based on the more clinically significant variant.

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