Fig 1.
Development of RSV-resistant near isogenic line YR32548-8-16 with details of markers used for foreground and background selection.
Numbers in parentheses indicate the total number of plants. SS: summer season, RGA: rapid generation advance in winter season, UN: Unkwang, HDS: Haedamssal.
Fig 2.
Foreground selection with gene specific marker InDel7 in BC1F1 and BC2F2.
M: 100 bp ladder, U: recurrent parent Unkwang, H: donor parent Haedamssal, *: selected plants.
Table 1.
Foreground selection by gene specific marker InDel7 in BC1F1, BC2F1 and BC2F2 populations.
Fig 3.
Background selection in BC2F1 and BC2F2.
A: Frequency of recurrent parent genome recovery in BC2F1, B: Frequency of recurrent parent recovery in BC2F2, C: Graphical genotype of selected lines in BC2F1 and in BC2F2 and their parents using KASP marker (a: recurrent parent Unkwang, b: donor parent Haedamssal, c: selected BC2F1 plant YR32548-8, d: the selected BC2F2 plant YR32548-8-16).
Fig 4.
Phenotypic differences between Unkwang and YR32548-8-16.
A: Booting stage, B: Ripening stage, C: Reaction to RSV disease, D: ELISA assay.