Fig 1.
Collection sites for An. albimanus larvae and adults.
Collections were performed in localities of the municipalities: San Antero in Córdoba Department, Atlantic Coast (09°23'36.8''N; 75° 46'11,8''W) and Buenaventura in Valle del Cauca Department, Pacific Coast (3°57′59.9″N; 77°22′42.7″W).
Fig 2.
Dendrogram based on Bayesian inference.
The cluster tree diagram that represents bacterial relationships was based in 16S rRNA gene sequences. Ureaplasma parvum was used as the outgroup. Colored circles represent members of bacterial genera. Paralel vertical lines from left to right define phylum, class and family. Branch support ranged between 0.93 and 1.
Table 1.
Taxonomic classification of bacterial gut isolates from Anopheles albimanus larvae and adults based on 16S rRNA gene sequences.
Fig 3.
Dendrogram based on Jaccard distances using the UPGMA algorithm with 1000 replicas.
Bacterial genera distribution according to locality and stage. The genera detected in the localities sampled in San Antero (Atlantic Coast) and Buenaventura (Pacific Coast) and in both. Larva and mosquito figures indicate the mosquito stage in which bacterial genera were detected.
Fig 4.
Venn diagram showing shared morphospecies among larvae, adults and localities, San Antero and Buenaventura.
The diagram shows the 28 morphospecies detected by locality and mosquito stage. In the intersections are the morphospecies shared by groups, locality and stage (represented by numbers).
Fig 5.
Morphospecies abundances in gut bacterial communities from larvae and adult Anopheles albimanus from San Antero and Buenaventura.
Morphospecies abundances are given in percentages, the values are shown at the right side of the bars; the accumulated frequency is also shown (left, Y axis). Colors represent morphospecies. (A) larvae form Buenaventura (Pacific Coast), (B) adults form Buenaventura, (C) larvae from San Antero (Atlantic Coast), (D) adults of San Antero.
Fig 6.
Morphospecies accumulation curves.
The X axis represents the number of samples, larvae or adults and the Y axis the number of morphospecies detected. Colored lines corresponds to the indexes evaluated by locality and stage. (A) San Antero (Atlantic Coast)—larvae, (B) San Antero—adults, (C) Buenaventura (Pacific Coast)—larvae, (D) Buenaventura—adults.
Table 2.
Alpha diversity indices for An. albimanus gut bacterial communities.
Fig 7.
The X axis shows the morphotypes of each genus; the Y axis, the percentage of antibiotic resistant and sensitive morphotypes. (S) Sensitivity, (R) resistance.