Fig 1.
Trilineage differentiation is seen in all groups of MSC treated with induction media.
Trilineage differentiation assays were performed on Standardbred, Thoroughbred, and blood-donor MSCs. Cells placed in induction media showed differentiation down adipogenic, osteogenic, and chondrogenic lines. Control MSCs cultured in media without induction agents showed no differentiation.
Fig 2.
Gating scheme for MSC selection used in flow cytometry.
Representative dot plots show the gating scheme that was used prior to quantification of MSCs positive and negative for the desired marker.
Fig 3.
Positive and negative cell populations for each antibody illustrate marker expression in MSCs.
A representative MSC sample from passage 2 shows MHC class II, CD44, CD90 and CD11a/18 expression.
Fig 4.
These graphs show the breed differences in marker expression over the time points (passages). Median marker expression is represented in each of the graphs as a percent of cells that show the marker as compared to the total gated cell population. The IQR is shown as the top and bottom of the box. Extreme values are shown with the error bar. Excluded data points are listed with a bullet. Passages with significantly different expression between the Thoroughbred and Standardbred populations are indicated by an asterisk.
Fig 5.
Marker expression by blood donor status.
These graphs show the differences in marker expression between blood donor horses and non-blood donor horses over successive passages. Median marker expression is represented in each of the graphs as a percent of cells that show the marker as compared to the total gated cell population. The IQR is shown as the top and bottom of the box. Extreme values are shown with the error bar. Excluded data points are listed with a bullet. Passages with significantly different expression between the Thoroughbred and Standardbred populations are indicated by an asterisk.
Table 1.
Correlation of marker expression.