Fig 1.
Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers.
Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the PCR products generated are 457bp for HPV-16 and 322bp for HPV-18.
Fig 2.
Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-31 E6/E7 primers.
Numbers are samples ID; L is a mid-range ladder while NC is the negative control. The size of the PCR products generated for HPV-31 is 263bp.
Fig 3.
Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-6 (334bp), 35 (358bp) and 33 (398bp) primers.
Numbers are samples positive to HPV 6, 35 and 33 as labelled; L is a mid-range ladder while NC is the negative control. The sizes of the PCR products generated are 334bp for HPV-6, 358bp for HPV-35 and 398bp for HPV-33.
Table 1.
Distribution of identified HPV genotypes and types of infection among individuals tested.
Table 2.
Distribution of HPV types by health status of individuals tested.
Table 3.
HPV E6 sequences of study isolates with their nucleotide substitutions and amino acid changes.
Table 4.
HPV E7 sequences of study isolates with their nucleotide substitutions and amino acid changes.
Fig 4.
Nucleotide sequence alignment of HPV-74 isolate with reference sequence and sequence from France, showing insertion of CCT.
The top row specifies the positions where variations were observed in the sequences; positions without variations were marked with an asterisk (*) while no asterisk indicated a variation position. The letters indicated their nucleotide base sequences.REF; Reference sequence.
Fig 5.
Phylogenetic tree of sequences of study isolates with HPV reference sequences.
All the nineteen study isolates clustered with only fourteen HPV reference sequences (REF). Study isolates are indicated by shaded triangles. *REF; Reference Sequences.
Fig 6.
Phylogenetic tree of HPV-16 isolates.
All the four HPV-16 study isolates clustered with African 2 variants on the phylogenetic tree. Study isolates are indicated by shaded triangles; AF1: African 1 variants; AFR2: African 2 variants.
Fig 7.
Phylogenetic tree of HPV-18 isolates.
The two HPV-18 study isolates clustered differently on the phylogenetic tree. Study isolates are indicated by shaded triangles.
Fig 8.
Phylogenetic tree of HPV-31 isolates.
The HPV-31 study isolate clustered with isolate from Portugal. The isolate is indicated by a shaded triangle.