Table 1.
Artificial seawater medium composition.
Table 2.
Initial major nutrient concentrations at the start of the experiment for the different species and culture media (N+ and N-).
Table 3.
Nutrient consumption by the end of the experiment (triplicate mean ± SD) for all species and treatments, with the corresponding two-way ANOVA results (n = 3).
Fig 1.
Growth curves expressed in cell.mL-1 of E. paludosa (A), N. alexandrina (B), and Staurosira sp. (C) as a function of time under different light (LL, ML, HL) and nitrogen (N+, N-) conditions.
Table 4.
Growth rate (μ in day-1), maximum cell concentration (A in log) and latency time (λ in day) (triplicate mean ± SD) for all species and culture conditions and the corresponding two-way ANOVA results (n = 3).
Fig 2.
Box and whisker plots for E. paludosa photosynthetic parameters: Maximum PSII quantum efficiency, Fv/Fm (A) and maximum light saturation parameter, Ek (B) for each culture condition and for two growth stages: Exponential growth phase (mid) and stationary phase (end).
Fig 3.
Box and whisker plots for N. alexandrina photosynthetic parameters: Maximum PSII quantum efficiency, Fv/Fm (A) and maximum light saturation parameter, Ek (B) for each culture condition and for two growth stages: Exponential growth phase (mid-growth) and stationary phase (end of growth).
Fig 4.
Box and whisker plots for Staurosira sp. photosynthetic parameters: Maximum PSII quantum efficiency, Fv/Fm (A) and maximum light saturation parameter, Ek (B) for each treatment and for two growth stages: Exponential growth phase (mid-growth) and stationary phase (end of growth).
Fig 5.
Box and whisker plots for E. paludosa (A), N. alexandrina (B), and Staurosira sp. (C) of Chl a content (pg.cells-1) and Carotenoid content (pg.cells-1) at the end of growth for each culture condition.
Fig 6.
Total lipid (A,B,C), carbohydrate (D,E,F), and protein ratios (G,H,I) of E. paludosa, N. alexandrina, and Staurosira sp. for each treatment.
* p < 0.05;**p < 0.01;***p < 0.001 for nitrogen conditions and $ p < 0.05;$ $p < 0.01;$ $ $p < 0.001 for light conditions.
Fig 7.
Biomass (A, B, C), lipid rate, and lipid production (D, E, F; high bar = lipid rate; low bar = lipid productivity) obtained at the end of the growth for E. paludosa, N. alexandrina, and Staurosira sp. for each treatment.
* p < 0.05; ** p < 0.01; *** p < 0.001 for nitrogen conditions and $ p < 0.05; $ $ p < 0.01; $ $ $ p < 0.001 for light conditions.
Table 5.
FAME composition of E. paludosa, N. alexandrina, and Staurosira sp. for all treatments.