Fig 1.
Fungal culture plates with bacterial contaminations.
Some common bacterial contaminations during culturing Magnaporthe oryzae Guy-11 strains on complete medium plates.
Fig 2.
Experimental procedure of CS method.
Fig 3.
Comparison the treatments basing on CS method and antibiotic inhibitions.
(A) M. oryzae Guy-11 strain mixed with E. coli or A. tumefaciens were treated using 4 different methods; (B) The treatments with M. oryzae 2539, 70–15, and TH3 strains mixed with A. tumefaciens. T1, CS treatment without antibiotics; T2, CS treatment in combination with antibiotics; T3, antibiotics only; T4, subculture on antibiotic-free media.
Fig 4.
PCR detection of bacterial DNA in progeny fungal colonies after the treatments.
PCR were performed using E. coli or A. tumefaciens specific primers and fungal DNA samples from M. oryzae Guy-11 strains contaminated with E. coli or A. tumefaciens (A) and M. oryzae 2539, 70–15, and TH3 strains contaminated with A. tumefaciens (B), as templates. O1-O5, the original contaminated strains before treatments; T1, CS treatment without antibiotics; T2, CS treatment in combination with antibiotics; T3, antibiotics only; T4, subculture on antibiotic-free media. The pure E. coli cultures (Ec) and A. tumefaciens (At) cultures were used as the positive controls, and the sterilized water (CK) as the negative controls. Mk, DNA Marker III (Tiangen Biotech Co.,Ltd, Beijing, China), a DNA ladder with bands in 4500, 3000, 2000, 1200, 800, 500 and 200 bp.
Fig 5.
CS treatments and antibiotics inhibition for removing bacteria from different fungal strains.
The naturally bacteria contaminated fungal strains of F. graminearum, C. gloeosporioides, R. solani, and B. rhodina were treated using the 4 different procedures; T1, CS treatment without antibiotics; T2, CS treatment in combination with antibiotics; T3, antibiotics only; T4, subculture on antibiotic-free media.