Table 1.
Demographic data of patients undergoing cystic echinococcosis surgery, histological features of the removed cyst, and molecular identification of Echinococcus granulosus in formalin-fixed paraffin embedded cyst tissue and serum by the cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit I (nad1) mitochondrial genes.
Fig 1.
Formalin-fixed paraffin embedded Echinococcus granulosus cyst stained with hematoxylin and eosin.
Cross-section of an Echinococcus granulosus cyst, the cyst wall and protoscoleces (A) 40×, (B) 100× and (C) 400× magnification. Arrows indicate the hooklets inside the protoscoleces D) Acellular laminated layers of the cyst wall 1000× magnification.
Table 2.
Genotypes of Echinococcus granulosus, identified in formalin-fixed paraffin embedded cyst tissue and serum by the cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit I (nad1) mitochondrial genes.
Table 3.
Genotype distribution of Echinococcus granulosus identified by the cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit I (nad1) mitochondrial genes according to the patient province of residency.
Fig 2.
Phylogram of Echinococcus granulosus sensu lato was inferred based on the nucleotide sequences of the cytochrome c oxidase I gene (cox1).
The evolutionary relationship of Echinococcus granulosus sensu lato was constructed by the neighbor-joining method, based on the nucleotide sequences of cox1 retrieved from this study (S1 Table) compared with reference sequences of E. granulosus sensu lato and other species of Echinococcus from GenBank, with Taenia saginata as outgroup. Bootstrap values obtained from 1000 replicates are indicated on branches in percentage, and only bootstraps values >70% are displayed. Evolutionary analyses were conducted in MEGA7.
Table 4.
Multiple alignment sequences of cytochrome c oxidase I (cox1) gene obtained from formalin-fixed paraffin embedded hydatid cyst tissue and patient serum with reference sequences retrieved from GenBank.
Fig 3.
Phylogram of Echinococcus granulosus sensu lato was inferred based on the nucleotide sequences of the NADH dehydrogenase subunit I gene (nad1).
The evolutionary relationship of Echinococcus granulosus sensu lato was constructed by the neighbor-joining method, based on the nucleotide sequences of nad1 retrieved from this study (S1 Table) compared with reference sequences of E. granulosus sensu lato and other species of Echinococcus from GenBank, with Taenia saginata as outgroup. Bootstrap values obtained from 1000 replicates are indicated on branches in percentage, and only bootstraps values >70% are displayed. Evolutionary analyses were conducted in MEGA7.
Table 5.
Multiple alignment sequences of NADH dehydrogenase subunit I (nad1) in formalin-fixed paraffin embedded hydatid cyst tissue and patient serum with reference sequences retrieved from GenBank.
Fig 4.
Phylogram of Echinococcus granulosus sensu lato was inferred based on the nucleotide sequences of concatenated cytochrome c oxidase I (cox1) and the NADH dehydrogenase subunit I (nad1).
The evolutionary relationship of Echinococcus granulosus sensu lato constructed by the neighbor-joining method, based on the nucleotide sequences of concatenated cox1 and nad1 retrieved from this study (S1 Table) compared with reference sequences of E. granulosus sensu lato and other species of Echinococcus from GenBank, with Taenia saginata as outgroup. Bootstrap values obtained from 1000 replicates are indicated on branches in percentage, and only bootstraps values >70% are displayed. Evolutionary analyses were conducted in MEGA7.