Table 1.
CWD-solution decontamination.
Fig 1.
RT-QuIC analysis of water or bleach treated CWD-positive brain homogenates.
A 10% brain homogenate of WTD-1 was mixed with 9 volumes of either water, 1%, or 10% bleach for 1 minute. Following treatment, the samples were diluted in RT-QuIC buffer to a 10−4 brain homogenate dilution and tested by RT-QuIC. Individual well data are shown for 4 replicate wells for the water treatment (A), and 8 replicate wells each for the 1% bleach treatment (B) and 10% bleach treatment (C). Note that the 1% bleach treated samples shown in B have longer lag times compared to the water treatment group in A, suggesting some inactivation occurred.
Fig 2.
RT-QuIC analysis of stainless-steel wires exposed to varying concentrations of WTD-1 (A) or MD-1 CWD-positive brain homogenates (B). Wires were immersed for 1 hour in CWD brain homogenate dilutions from 10−1 to 10−8. Following immersion wires were washed, dried, and later placed directly into RT-QuIC reaction wells. As negative controls, wires were immersed in normal deer brain homogenate (NBH) at 10−2 and shown in panel A and untreated wires are shown in panel B. Each curve shown is the average fluorescence of 4 wells tested per dilution. The dilutions are shown with different symbols, and labeled to the right of each curve.
Table 2.
One-minute bleach treatments of CWD-coated wires.
Table 3.
Five-minute bleach treatments of CWD-coated wires.
Fig 3.
RT-QuIC analysis for treatments of WTD-1 coated wires.
All wires were exposed to 10−2 WTD-1 CWD-positive brain homogenate, washed and allowed to dry before treatment. Wires were then immersed in either water or bleach treatments, briefly rinsed and dried before being placed into RT-QuIC plate wells for analysis of seeding activity. Bleach concentrations were tested at 1% (panels C, D), 10% (panels E, F) and 40% (panels G, H) for either 1 minute (panels C, E, G) or 5 minutes (panels D, F, H). Untreated WTD-1 coated wires are shown in panel A as a positive control. Water treated wires are shown in panel B. Individual well data are shown, 4 wells were tested for untreated (panel A) and water treated wires (panel B), 8 wires were tested for each bleach condition (panels C-H).
Table 4.
Bleach treatment of CWD-infected transgenic mouse brain.