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Fig 1.

Principal Coordinate Analysis (PCoA) using weighted UniFrac distances of A & B) algal sequences and C & D) bacterial sequences for all field samples by year (2011, 2012 and 2013), and region (patch vs non-patch) and size fraction (>0.2 and >5 micron).

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Fig 1 Expand

Fig 2.

Class- (left panel) and genus/species- (right panel) level relative abundances of algal sequences from size fractionated (>0.2 and >5 micron) samples collected within and immediately adjacent (<10m) to the Cochlodinium bloom patches, designated ‘patch’ (P) and ‘non-patch’ (NP) samples, respectively, for years 2011, 2012 and 2013.

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Fig 2 Expand

Fig 3.

Venn diagrams demonstrating the shared and unique A) 18S OTUs-, C) 16S OTUs- and average abundances of corresponding B) 18S consensus lineages- and D) 16S consensus lineages- of the core microbiomes found among Patch >0.2 and >5, and Non-patch >0.2 and >5 micron size fractioned samples. Venn diagrams colors represent the following: patch >0.2 (blue), patch >5 (yellow), non-patch >0.2 (green) and non-patch >5 (red). Numbers within the diagrams represent number of OTUs.

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Fig 3 Expand

Fig 4.

Principal component analyses using Phylum- and genus/species- level Hellinger transformed relative abundances of bacterial sequences from size fractionated (>0.2 and >5 micron) samples collected within and immediately adjacent (<10m) to the Cochlodinium bloom patches, designated ‘patch’ (P) and ‘non-patch’ (NP) samples, respectively, for years 2011, 2012 and 2013.

Percent variation explained by each principal component is indicated in parentheses.

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Fig 4 Expand

Fig 5.

Phylum- (left panel) and Order- (right panel) level relative abundances of bacterial sequences from size fractionated (>0.2 and >5 micron) samples collected within and immediately adjacent (<10m) to the Cochlodinium bloom patches, designated ‘patch’ (P) and ‘non-patch’ (NP) samples, respectively, for years 2011, 2012 and 2013.

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Fig 5 Expand

Fig 6.

Genus- level relative abundances of bacterial sequences from size fractionated (>0.2 and >5 micron) samples collected within and immediately adjacent (<10m) to the Cochlodinium bloom patches, designated ‘patch’ (P) and ‘non-patch’ (NP) samples, respectively, for years 2011, 2012 and 2013.

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Fig 6 Expand

Fig 7.

Relative abundances of algal (18S) sequences for the experimental control, and the Cochlodinium polykrikoides culture whole cell addition treatment during an experiment conducted using the natural phytoplankton community of Old Fort Pond, NY.

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Fig 7 Expand