Fig 1.
Microarray signal intensity distribuiton before and after normalization.
Raw (a) and normalized (b) microarray probe M values obtained for eight different individuals using three different blood collections systems. Colors represent individual donors.
Fig 2.
Principal component analysis of technical replicates.
Principal Component Analysis (PCA) of normalized mRNA transcript values across individuals (colors) and platforms.
Table 1.
Comparison of within and across donor probe M value correlations using three different blood collection systems.
Fig 3.
Modest positive trend of increased probe variance and GC content.
Scatter plot showing RNAgard® probe variance by GC%. Red line shows linear regression fit.
Table 2.
Correlation of within donor and preservation method probe error with probe properties.
Table 3.
Biological processes as indicated by specific Gene Ontology Id’s most highly associated with variable mRNA within RNAgard® and PAXgene®RNA preservation methods.
Table 4.
Biological processesfunctions as indicated by specific Gene Ontology Id’s Gene Ontology terms associated with differentially expressed genes in the RNAgard® and PAXgene®RNA systems.